Cassia mimosoides L. decoction improves non-alcoholic fatty liver disease by modulating the pregnane X receptor.

IF 4.8 2区 医学 Q1 CHEMISTRY, MEDICINAL
Jian Zhao, Yanhua Zhong, Qingyin Huang, Zhisen Pan, Yi Zheng, Deyu Miao, Siqi Liu, Penglong Chen, Changhui Liu, Min Liu, Chuangpeng Shen
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引用次数: 0

Abstract

Ethnopharmacological relevance: Cassia mimosoides L. (CML) is a traditional Chinese medicine (TCM), which is frequently used in the clinical practice of TCM in the Lingnan region of China for the treatment of obesity. However, it is not clear whether decoction of cassia seeds has beneficial effects on non-alcoholic fatty liver disease (NAFLD).

Objectives: This study investigates the effect of CML on NAFLD and its underlying mechanisms.

Materials and methods: The high-fat diet (HFD) was used to induce NAFLD mice, and 40 male C57BL/6J mice were divided into Control, HFD, and CML groups (CML-low 1.5 g/kg, CML-medium 2.25 g/kg, CML-high 4.5 g/kg). The mouse primary hepatocytes (MPHs) of wild type (WT) and PXR-/- mice were induced using OAPA and divided into Control, OAPA, and CML groups (10 mg/L, 100 mg/L). Glycolipid metabolism, inflammation, and oxidative stress levels were detected in vivo and in vitro.

Results: Compared to the HFD group, the CML groups demonstrated reduced body weight, triglycerides, total cholesterol, blood glucose, and mRNA levels of the lipid metabolism genes Srebp-1c and ACC1 in mice (p < 0.05 or 0.01). The ELISA results indicated that CML inhibited the production of IL-1β, IL-6, and TNF-α (p < 0.05). Furthermore, CML increased the SOD level (p < 0.01) to improve oxidative stress. RNA-seq expression showed that CML suppressed the transcriptional level of pregnane X receptor (PXR)(p < 0.05). In vitro experiments, the protective effect of CML against OAPA-induced lipid accumulation and inflammation observed in WT MPHs disappeared in PXR-/- MPHs (IC50: 1.04 mg/mL).

Conclusion: CML decoction ameliorates NAFLD mainly by inhibiting the PXR signaling pathway, which provides a theoretical basis for the broad application of CML in clinical practice.

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来源期刊
Journal of ethnopharmacology
Journal of ethnopharmacology 医学-全科医学与补充医学
CiteScore
10.30
自引率
5.60%
发文量
967
审稿时长
77 days
期刊介绍: The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.
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