Genomic epidemiology and genetic characteristics of clinical Campylobacter species cocirculating in West Bengal, India, 2019, using whole genome analysis.
{"title":"Genomic epidemiology and genetic characteristics of clinical <i>Campylobacter</i> species cocirculating in West Bengal, India, 2019, using whole genome analysis.","authors":"Daichi Morita, Asish Kumar Mukhopadhyay, Goutam Chowdhury, Fumito Maruyama, Miyuki Kanda, Yuki Yamamoto, Hidetoshi Tahara, Piyali Mukherjee, Mainak Bardhan, Takanori Kumagai, Kei Kitahara, Shin-Ichi Miyoshi, Shanta Dutta, Teruo Kuroda","doi":"10.1128/aac.01108-24","DOIUrl":null,"url":null,"abstract":"<p><p><i>Campylobacter</i> species are the most common pathogens responsible for foodborne gastroenteritis worldwide. India is a region with frequent diarrheal infections and a high level of <i>Campylobacter</i> infection incidence, but the detailed genomic information is limited. This study aimed to characterize 112 isolates of <i>Campylobacter</i> from diarrhea patients at two hospitals in Kolkata, West Bengal, by whole genome analysis. The <i>Campylobacter</i> isolates consisted of 90 <i>C</i>. <i>jejuni</i>, 20 <i>C</i>. <i>coli</i>, and 2 <i>C</i>. <i>lari</i> isolates. Multilocus sequence typing analysis revealed that the largest sequence type (ST) populations were ST-2131 in <i>C. jejuni</i> and ST-830 in <i>C. coli</i> and seven novel STs were found in <i>C. jejuni</i> and one in <i>C. coli</i>. Notably, ST-2131, which is rarely seen elsewhere, was positive for a sialylated LOS-related gene (<i>wlaN +neuA + cstIII</i>) associated with Guillain-Barré syndrome. Antibiotic resistance factors predicted from the genome sequence included <i>blaOXA</i> variants (58.9%), <i>tet(O)</i> (54.5%), <i>tet(W)</i> (0.9%), <i>ant(6)-Ia</i> (0.9%), mutation in GyrA (T86I, T86I+D90N, T86I+P104S, T86I+D90<i>N</i>+P104S) (79.5%), and mutation in 23S rRNA (A2075G) (12.5%). In addition to the high drug resistance of <i>Campylobacter</i> in Kolkata, <i>Campylobacter</i> pathogens were circulating that may be associated with Guillain-Barré syndrome. This study indicates the importance of genomic analysis in the surveillance of pathogens, which provides genomic information on genetic diversity, virulence mechanisms, and determinants of antimicrobial resistance.</p>","PeriodicalId":8152,"journal":{"name":"Antimicrobial Agents and Chemotherapy","volume":" ","pages":"e0110824"},"PeriodicalIF":4.1000,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antimicrobial Agents and Chemotherapy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1128/aac.01108-24","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Campylobacter species are the most common pathogens responsible for foodborne gastroenteritis worldwide. India is a region with frequent diarrheal infections and a high level of Campylobacter infection incidence, but the detailed genomic information is limited. This study aimed to characterize 112 isolates of Campylobacter from diarrhea patients at two hospitals in Kolkata, West Bengal, by whole genome analysis. The Campylobacter isolates consisted of 90 C. jejuni, 20 C. coli, and 2 C. lari isolates. Multilocus sequence typing analysis revealed that the largest sequence type (ST) populations were ST-2131 in C. jejuni and ST-830 in C. coli and seven novel STs were found in C. jejuni and one in C. coli. Notably, ST-2131, which is rarely seen elsewhere, was positive for a sialylated LOS-related gene (wlaN +neuA + cstIII) associated with Guillain-Barré syndrome. Antibiotic resistance factors predicted from the genome sequence included blaOXA variants (58.9%), tet(O) (54.5%), tet(W) (0.9%), ant(6)-Ia (0.9%), mutation in GyrA (T86I, T86I+D90N, T86I+P104S, T86I+D90N+P104S) (79.5%), and mutation in 23S rRNA (A2075G) (12.5%). In addition to the high drug resistance of Campylobacter in Kolkata, Campylobacter pathogens were circulating that may be associated with Guillain-Barré syndrome. This study indicates the importance of genomic analysis in the surveillance of pathogens, which provides genomic information on genetic diversity, virulence mechanisms, and determinants of antimicrobial resistance.
期刊介绍:
Antimicrobial Agents and Chemotherapy (AAC) features interdisciplinary studies that build our understanding of the underlying mechanisms and therapeutic applications of antimicrobial and antiparasitic agents and chemotherapy.