Impact of Warm Ischemia Time on HER2 Expression in Breast Cancer Surgical Specimens.

IF 1.6 4区 医学 Q4 ONCOLOGY
Nobuyasu Suganuma, Yuka Matsubara, Akari Takahashi, Takashi Yamanaka, Toshinari Yamashita, Emi Yoshioka, Kae Kawachi, Tomonori Yokose, Hiroto Narimatsu, Daisuke Hoshino, Yohei Miyagi, Aya Saito
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引用次数: 0

Abstract

Background/aim: Tissue sample quality control has become increasingly crucial as these samples are integral to basic research and clinical practice, particularly in immunohistochemistry and gene panel testing. Standard PREanalytical Code (SPREC) was developed to standardize pre-analytical processes, including warm ischemia time (WIT), cold ischemia time (CIT), and fixation time (FT), which can influence the surgical specimen quality. This study investigated the impact of WIT, CIT, and FT on estrogen receptor (ER), progesterone receptor (PgR), and human epidermal growth factor receptor 2 (HER2) expression in breast cancer (BC) surgical specimens.

Patients and methods: We enrolled 277 patients with first-time BC who underwent surgery at Kanagawa Cancer Center between May 2018 and April 2019. WIT, CIT, and FT were recorded using SPREC ver. 3.0, and their effects on ER, PgR, and HER2 expression were analyzed using immunohistochemistry. Surgical specimens were compared with preoperative needle biopsy samples from the same tumors to control for WIT, CIT, and FT variability.

Results: The median WIT, CIT, and FT were 23 min, 37 min, and 43 h, respectively. Compliance with the American Society of Clinical Oncology/College of American Pathologists guidelines was 91.7% for CIT and 94.9% for FT. ER and PgR expression in surgical specimens decreased with prolonged CIT and FT, but differences were non-significant. However, HER2 expression increased significantly when WIT exceeded 30 min.

Conclusion: WIT could significantly influence HER2 expression in BC surgical specimens, highlighting the need for meticulous WIT control during BC surgery to ensure accurate HER2 assessment, which is critical for guiding therapeutic decisions.

热缺血时间对乳腺癌手术标本中HER2表达的影响
背景/目的:组织样本质量控制变得越来越重要,因为这些样本是基础研究和临床实践不可或缺的一部分,特别是在免疫组织化学和基因面板检测中。为了规范影响手术标本质量的前分析过程,包括热缺血时间(WIT)、冷缺血时间(CIT)和固定时间(FT),制定了标准前分析规范(SPREC)。本研究探讨了WIT、CIT和FT对乳腺癌手术标本中雌激素受体(ER)、孕激素受体(PgR)和人表皮生长因子受体2 (HER2)表达的影响。患者和方法:我们招募了277名首次患有BC的患者,他们于2018年5月至2019年4月在神奈川癌症中心接受了手术。采用SPREC软件记录WIT、CIT、FT。采用免疫组化方法分析其对ER、PgR、HER2表达的影响。将手术标本与术前同一肿瘤的穿刺活检标本进行比较,以对照WIT、CIT和FT的可变性。结果:中位WIT、CIT和FT分别为23分钟、37分钟和43小时。CIT和FT对美国临床肿瘤学会/美国病理学家学会指南的依从性分别为91.7%和94.9%。手术标本中ER和PgR表达随CIT和FT的延长而降低,但差异无统计学意义。结论:WIT可以显著影响BC手术标本中HER2的表达,强调在BC手术过程中需要严格控制WIT,以确保准确的HER2评估,这对指导治疗决策至关重要。
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来源期刊
Anticancer research
Anticancer research 医学-肿瘤学
CiteScore
3.70
自引率
10.00%
发文量
566
审稿时长
2 months
期刊介绍: ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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