Analysis of Methylation of Tumor-suppressive miRNAs and KRAS/TP53 Mutations in Pancreatic Juice.

IF 1.6 4区医学 Q4 ONCOLOGY

Anticancer research Pub Date : 2024-12-01 DOI:10.21873/anticanres.17353

Koushiro Ohtsubo, Kunio Miyake, Shigeki Sato, Hiroyuki Sakaguchi, Koji Fukuda, Hiroshi Kotani, Akihiro Nishiyama, Shigeki Nanjo, Kaname Yamashita, Shinji Takeuchi, Seiji Yano, Sawako Kuruma, Jun Nakahodo, Masataka Kikuyama, Hiroaki Taniguchi

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引用次数: 0

Abstract

Background/aim: We previously reported the usefulness of detecting aberrant methylation in tumor suppressive microRNAs (miRNAs) in bile and plasma to discriminate pancreaticobiliary cancers from benign pancreaticobiliary diseases. This study analyzed the methylation of miRNAs in pancreatic juice to identify those specific to pancreatic cancer (PC).

Patients and methods: Pancreatic juice was collected from 20 patients with PC, including eight with intraductal papillary mucinous carcinoma (IPMC), two with low grade-pancreatic intraepithelial neoplasia (LG-PanIN), 32 with LG-intraductal papillary mucinous neoplasm (IPMN), and seven with benign pancreatic lesions. Polymerase chain reaction amplification and sequencing were performed for three tumor suppressive miRNAs (miR-200a, 200b, and 1247), and their methylation rates were determined. Additionally, KRAS and TP53 mutations were analyzed.

Results: The methylation rate of miR-1247 was significantly higher in patients with PC than in those with LG-PanIN/IPMN. Furthermore, it was significantly higher in patients with IPMC than in those with LG-IPMN. KRAS and TP53 mutations were detected in seven (70%) and one (10%) of the patients with PC, respectively.

Conclusion: Analyzing the methylation of miR-1247 in addition to KRAS and TP53 mutations in pancreatic juice may be useful to distinguish PC from PanIN/IPMN.

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.