Synthesis, Structural Characterization, and Antimicrobial Evaluation of Silver Nanoparticle-embedded Layered Double Hydroxides for Delivery in Polymeric Hydrogel Matrices

IF 2.7 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmaceutical Innovation Pub Date : 2024-12-02 DOI:10.1007/s12247-024-09901-2

Sabna Kotta, Hibah Mubarak Aldawsari, Nabil A. Alhakamy, Mahmoud Abdelkhalek Elfaky, Shaimaa M. Badr-Eldin

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Abstract

Purpose

This study aimed to synthesize and characterize silver nanoparticle-embedded layered double hydroxides (Ag-LDH) nanohybrids for potential antimicrobial applications. The research also focused on identifying suitable polymeric hydrogel matrices for embedding the Ag-LDH nanohybrids.

Methods

Ag-LDH nanohybrids were synthesized and the resulting nanohybrids underwent comprehensive characterization using various techniques, including UV-Vis spectroscopy, FTIR spectroscopy, XRD, TGA, DSC, DTA, particle size analysis, zeta potential measurement, and transmission electron microscopy. The antimicrobial activity of the LDH, Ag-NPs, and Ag-LDH was evaluated against a panel of Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and a fungus (Candida albicans) using the agar diffusion method. Polymeric hydrogel matrices, including sodium carboxymethyl cellulose, hydroxypropyl methylcellulose, Carbopol 940, polyvinyl alcohol, and chitosan, were prepared and characterized for their rheological properties using a viscometer.

Results

Characterization techniques confirmed the successful synthesis and intercalation of Ag-NPs within the LDH structure. Antimicrobial studies demonstrated enhanced antibacterial and antifungal activities of the Ag-LDH nanohybrid compared to individual LDH and Ag-NPs. The inhibition zone diameters for Ag-LDH against S. aureus, B. subtilis, E. coli, P. aeruginosa, and C. albicans were 16 ± 0.4 mm, 21 ± 0.8 mm, 16.8 ± 0.7 mm, 19.1 ± 1.1 mm, and 21.3 ± 0.9 mm, respectively. Rheological profiling of the Ag-LDH embedded polymeric matrices revealed varying viscosity profiles, indicating different flow behaviors and potential suitability for various delivery approaches.

Conclusion

The study successfully synthesized, characterized, and demonstrated the enhanced antimicrobial activity of Ag-LDH nanohybrids. Polymeric hydrogel matrices suitable for embedding these nanohybrids were also identified and characterized. Further research, including preclinical studies, could lead to the development of a scalable Ag-LDH hybrid suitable for clinical practice.

Graphical Abstract

Abstract Image

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纳米银嵌入层状双氢氧化物的合成、结构表征和抗菌评价

目的合成并表征银纳米粒子嵌入的层状双氢氧化物（Ag-LDH）纳米杂化物，并将其应用于抗菌领域。研究还着重于寻找合适的聚合物水凝胶基质来包埋Ag-LDH纳米杂化物。方法采用UV-Vis光谱、FTIR光谱、XRD、TGA、DSC、DTA、粒度分析、zeta电位测量、透射电镜等技术对合成的sag - ldh纳米杂交物进行了综合表征。采用琼脂扩散法对革兰氏阳性菌（金黄色葡萄球菌和枯草芽孢杆菌）、革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）和真菌（白色念珠菌）的抑菌活性进行了评估。制备了羧甲基纤维素钠、羟丙基甲基纤维素、卡波波尔940、聚乙烯醇和壳聚糖等聚合物水凝胶基质，并用粘度计对其流变性能进行了表征。结果表征技术证实了Ag-NPs的成功合成和嵌入LDH结构。抗菌研究表明，与单个LDH和Ag-NPs相比，Ag-LDH纳米杂化物的抗菌和抗真菌活性增强。Ag-LDH对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌、铜绿假单胞菌和白色念珠菌的抑制带直径分别为16±0.4 mm、21±0.8 mm、16.8±0.7 mm、19.1±1.1 mm和21.3±0.9 mm。Ag-LDH嵌入聚合物基质的流变学分析显示了不同的粘度分布，表明不同的流动行为和不同的输送方式的潜在适用性。结论成功合成、表征并证明了Ag-LDH纳米杂种具有较强的抗菌活性。此外，还鉴定了适合于包埋这些纳米杂化物的聚合物水凝胶基质。进一步的研究，包括临床前研究，可能会导致适合临床实践的可扩展的Ag-LDH混合物的发展。图形抽象

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来源期刊

Journal of Pharmaceutical Innovation PHARMACOLOGY & PHARMACY-

CiteScore

3.70

自引率

3.80%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Pharmaceutical Innovation (JPI), is an international, multidisciplinary peer-reviewed scientific journal dedicated to publishing high quality papers emphasizing innovative research and applied technologies within the pharmaceutical and biotechnology industries. JPI''s goal is to be the premier communication vehicle for the critical body of knowledge that is needed for scientific evolution and technical innovation, from R&D to market. Topics will fall under the following categories: Materials science, Product design, Process design, optimization, automation and control, Facilities; Information management, Regulatory policy and strategy, Supply chain developments , Education and professional development, Journal of Pharmaceutical Innovation publishes four issues a year.