A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging.

IF 2.2 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Tomography Pub Date : 2024-11-20 DOI:10.3390/tomography10110135

Sophia Trozzo, Bijita Neupane, Paula J Foster

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引用次数: 0

Abstract

Background: Preclinical cell tracking is enhanced with a multimodal imaging approach. Bioluminescence imaging (BLI) is a highly sensitive optical modality that relies on engineering cells to constitutively express a luciferase gene. Magnetic particle imaging (MPI) is a newer imaging modality that directly detects superparamagnetic iron oxide (SPIO) particles used to label cells. Here, we compare BLI and MPI for imaging cells in vitro and in vivo.

Methods: Mouse 4T1 breast carcinoma cells were transduced to express firefly luciferase, labeled with SPIO (ProMag), and imaged as cell samples after subcutaneous injection into mice.

Results: For cell samples, the BLI and MPI signals were strongly correlated with cell number. Both modalities presented limitations for imaging cells in vivo. For BLI, weak signal penetration, signal attenuation, and scattering prevented the detection of cells for mice with hair and for cells far from the tissue surface. For MPI, background signals obscured the detection of low cell numbers due to the limited dynamic range, and cell numbers could not be accurately quantified from in vivo images.

Conclusions: It is important to understand the shortcomings of these imaging modalities to develop strategies to improve cellular detection sensitivity.

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磁粉成像和生物发光成像的灵敏度和细胞检测能力比较。

背景：临床前细胞追踪可通过多模态成像方法得到加强。生物发光成像（BLI）是一种高灵敏度的光学模式，依赖于工程细胞组成性表达荧光素酶基因。磁粉成像（MPI）是一种较新的成像模式，可直接检测用于标记细胞的超顺磁性氧化铁（SPIO）颗粒。在此，我们比较了 BLI 和 MPI 在体外和体内对细胞成像的效果：方法：转导小鼠 4T1 乳腺癌细胞以表达萤火虫荧光素酶，用 SPIO（ProMag）标记，皮下注射到小鼠体内后作为细胞样本成像：对于细胞样本，BLI 和 MPI 信号与细胞数量密切相关。这两种成像模式对体内细胞成像都有局限性。就 BLI 而言，信号穿透力弱、信号衰减和散射阻碍了对有毛发的小鼠和远离组织表面的细胞的检测。就 MPI 而言，由于动态范围有限，背景信号掩盖了对低细胞数的检测，而且无法从体内图像中准确量化细胞数：结论：了解这些成像模式的缺点对制定提高细胞检测灵敏度的策略非常重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tomography Medicine-Radiology, Nuclear Medicine and Imaging

CiteScore

2.70

自引率

10.50%

发文量

222

期刊介绍： TomographyTM publishes basic (technical and pre-clinical) and clinical scientific articles which involve the advancement of imaging technologies. Tomography encompasses studies that use single or multiple imaging modalities including for example CT, US, PET, SPECT, MR and hyperpolarization technologies, as well as optical modalities (i.e. bioluminescence, photoacoustic, endomicroscopy, fiber optic imaging and optical computed tomography) in basic sciences, engineering, preclinical and clinical medicine. Tomography also welcomes studies involving exploration and refinement of contrast mechanisms and image-derived metrics within and across modalities toward the development of novel imaging probes for image-based feedback and intervention. The use of imaging in biology and medicine provides unparalleled opportunities to noninvasively interrogate tissues to obtain real-time dynamic and quantitative information required for diagnosis and response to interventions and to follow evolving pathological conditions. As multi-modal studies and the complexities of imaging technologies themselves are ever increasing to provide advanced information to scientists and clinicians. Tomography provides a unique publication venue allowing investigators the opportunity to more precisely communicate integrated findings related to the diverse and heterogeneous features associated with underlying anatomical, physiological, functional, metabolic and molecular genetic activities of normal and diseased tissue. Thus Tomography publishes peer-reviewed articles which involve the broad use of imaging of any tissue and disease type including both preclinical and clinical investigations. In addition, hardware/software along with chemical and molecular probe advances are welcome as they are deemed to significantly contribute towards the long-term goal of improving the overall impact of imaging on scientific and clinical discovery.