The Utility of BSND Immunohistochemistry in the Differential Diagnosis of Oncocytic and Warthin-like Mucoepidermoid Carcinoma of Salivary Gland.

IF 3.2 Q2 PATHOLOGY

Head & Neck Pathology Pub Date : 2024-11-26 DOI:10.1007/s12105-024-01728-0

Bin Xu, Achim Jungbluth, Denise Frosina, Anas Alabkaa, Rene Serrette, Howard Qin, Dibisha Roy, Ronald Ghossein, Nora Katabi

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引用次数: 0

Abstract

Purpose: BSND is a chloride channel subunit that is expressed in the normal salivary gland. We aimed to validate the utility of BSND immunohistochemistry in the differential diagnosis of oncocytic salivary gland neoplasms.

Methods: BSND immunohistochemistry was performed in a retrospective cohort of 93 salivary gland lesions, enriched with tumors with oncocytic features and histologic variants of mucoepidermoid carcinoma (MEC).

Results: All oncocytomas (n = 18) showed diffuse membranous BSND immunopositivity. Warthin tumors (n = 18) were also positive for BSND, but the staining pattern was patchy cytoplasmic and membranous in 10-25% of tumor cells. Using a threshold of 10% BSND-positive cells, all Warthin tumors were positive, while none of Warthin-like MECs or non-MEC salivary tumors were positive. Applying the same 10% positivity criterion, two oncocytic MECs were positive for BSND. The percentage of BSND staining in oncocytic MECs was up to 20%. In contrast, BSND was diffusely positive in oncocytomas with a median percentage of positivity of 95% (range: 40 - 100%). Therefore, a higher threshold of > 20% BSND-positive cells may be considered when differentiating between oncocytoma and oncocytic MEC.

Conclusion: BSND immunohistochemistry is a potentially useful diagnostic marker for salivary gland neoplasms, especially oncocytic and Warthin-like MECs. A threshold of ≥ 10% positivity can differentiate Warthin tumors from Warthin-like MECs, whereas > 20% positivity can be effective for separating oncocytomas from oncocytic MECs.

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BSND 免疫组化技术在唾液腺肿瘤细胞癌和疣状黏液表皮样癌鉴别诊断中的应用。

目的：BSND是一种在正常唾液腺中表达的氯离子通道亚基。我们旨在验证 BSND 免疫组化在肿瘤性唾液腺肿瘤鉴别诊断中的实用性：方法：我们对 93 例唾液腺病变进行了 BSND 免疫组化，其中包括具有肿瘤细胞特征的肿瘤和粘液表皮样癌（MEC）的组织学变体：结果：所有肿瘤细胞瘤（18 例）均显示弥漫性膜性 BSND 免疫阳性。疣状肿瘤（18 个）的 BSND 也呈阳性，但染色模式为斑片状胞质染色，10%-25% 的肿瘤细胞呈膜性染色。以10%的BSND阳性细胞为阈值，所有Warthin肿瘤均呈阳性，而Warthin样MECs或非MEC唾液肿瘤均不呈阳性。采用同样的 10%阳性标准，有两个肿瘤细胞 MEC 的 BSND 呈阳性。肿瘤细胞 MEC 中 BSND 染色的比例高达 20%。相比之下，BSND 在肿瘤细胞瘤中呈弥漫性阳性，阳性率中位数为 95%（范围：40 - 100%）。因此，在区分肿瘤细胞瘤和肿瘤细胞 MEC 时，可考虑将 BSND 阳性细胞的比例提高到 20% 以上：结论：BSND免疫组化是唾液腺肿瘤，尤其是肿瘤细胞瘤和Warthin样MEC的一种潜在有用的诊断标志物。≥10%的阳性阈值可将Warthin肿瘤与Warthin样MEC区分开来，而>20%的阳性可有效区分肿瘤细胞瘤与肿瘤细胞性MEC。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Head & Neck Pathology PATHOLOGY-

CiteScore

5.70

自引率

9.50%

发文量

期刊介绍： Head & Neck Pathology presents scholarly papers, reviews and symposia that cover the spectrum of human surgical pathology within the anatomic zones of the oral cavity, sinonasal tract, larynx, hypopharynx, salivary gland, ear and temporal bone, and neck. The journal publishes rapid developments in new diagnostic criteria, intraoperative consultation, immunohistochemical studies, molecular techniques, genetic analyses, diagnostic aids, experimental pathology, cytology, radiographic imaging, and application of uniform terminology to allow practitioners to continue to maintain and expand their knowledge in the subspecialty of head and neck pathology. Coverage of practical application to daily clinical practice is supported with proceedings and symposia from international societies and academies devoted to this field. Single-blind peer review The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.