Ahmed S Fahad, Matías F Gutiérrez-Gonzalez, Bharat Madan, Brandon J DeKosky
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引用次数: 0
Abstract
Antibody repertoire sequencing and display library screening are powerful approaches for antibody discovery and engineering that can connect DNA sequence with antibody function. Antibody display and screening studies have made a tremendous impact on immunology and biotechnology over the last decade, accelerated by technological advances in high-throughput DNA sequencing techniques. Indeed, bioinformatic analysis of antibody DNA library data has now taken a central role in modern antibody drug discovery, and is also critical for many ongoing studies of human immune development. Here, we describe current trends in antibody DNA library screening and analysis, and introduce a selection of protocols describing fundamental bioinformatic techniques to enable scientists to efficiently study antibody DNA libraries.
抗体复合物测序和展示文库筛选是发现抗体和进行抗体工程的有力方法，可将 DNA 序列与抗体功能联系起来。在过去十年中，由于高通量 DNA 测序技术的进步，抗体展示和筛选研究对免疫学和生物技术产生了巨大影响。事实上，对抗体 DNA 文库数据进行生物信息学分析已在现代抗体药物发现中占据了核心地位，而且对许多正在进行的人类免疫发展研究也至关重要。在这里，我们将介绍当前抗体 DNA 文库筛选和分析的趋势，并介绍一些描述基本生物信息学技术的方案，使科学家们能够高效地研究抗体 DNA 文库。
Cold Spring Harbor protocolsBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.00
自引率
0.00%
发文量
163
期刊介绍：
Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.
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