Characterization of Multipurpose Isolate Pseudomonas aeruginosa AMBCD-1 for Biocontrol and Biodegradation Efficiency

IF 1.5 Q4 ENGINEERING, ENVIRONMENTAL

Environmental Quality Management Pub Date : 2024-11-26 DOI:10.1002/tqem.70000

Tanu Singh, Safina Ismail, Kishan Kumar Raj, Anup Kumar Singh, Tapas Chowdhury, Ravindra Soni

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引用次数: 0

Abstract

The goal of the study was to assess the capability of the bacteria Pseudomonas aeruginosa AMBCD-1 for paddy straw degradation as well as its biological control abilities against the plant wilt pathogen Fusarium. Fourier-transform infrared spectroscopy (FTIR) data showed that AMBCD-1 culture-inoculated treatments dramatically reduced the levels of cellulose, lignin, and hemicelluloses in the deteriorated paddy straw. Additionally, a dual culture technique was utilized to investigate antagonistic activity in vitro using nutritional agar media and potato dextrose agar in equal proportions (1:1) for suppressing the growth of the fungus Fusarium oxysporum and Sclerotium rolfsii in order to assess the biocontrol properties of the chosen bacteria. Following incubation, it was shown that AMBCD-1 exhibited a 66.67% antibacterial activity against the fungus Fusarium oxysporum. Furthermore, the pyocyanin pigment was also extracted from the P. aeruginosa AMBCD-1 strain and confirmed at a mass value of 211.2 using liquid chromatography mass spectrometry (LC-MS), with its standard peak observed at 1.48 min. The results revealed that bacterium P. aeruginosa AMBCD-1 had the ability to biodegrade paddy straw and also inhibit the appearance of the wilt disease caused by Fusarium species in numerous crops.

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多用途铜绿假单胞菌 AMBCD-1 的生物防治和生物降解效率表征

这项研究的目的是评估铜绿假单胞菌 AMBCD-1 降解稻草的能力及其对植物枯萎病病原体镰刀菌的生物防治能力。傅立叶变换红外光谱（FTIR）数据显示，AMBCD-1 培养液接种处理可显著降低变质稻草中纤维素、木质素和半纤维素的含量。此外，为了评估所选细菌的生物防治特性，还采用了双重培养技术，以等比例（1:1）使用营养琼脂培养基和马铃薯葡萄糖琼脂在体外研究拮抗活性，以抑制真菌镰刀菌（Fusarium oxysporum）和球孢霉（Sclerotium rolfsii）的生长。培养结果表明，AMBCD-1 对真菌 Fusarium oxysporum 的抗菌活性为 66.67%。此外，还从铜绿微囊藻 AMBCD-1 菌株中提取出了焦花青素色素，并通过液相色谱质谱法（LC-MS）确认其质量值为 211.2，其标准峰出现在 1.48 分钟处。结果表明，铜绿微囊藻 AMBCD-1 菌株具有生物降解稻草的能力，还能抑制由镰刀菌引起的多种作物枯萎病的发生。

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来源期刊

Environmental Quality Management Environmental Science-Management, Monitoring, Policy and Law

CiteScore

2.20

自引率

0.00%

发文量

期刊介绍： Four times a year, this practical journal shows you how to improve environmental performance and exceed voluntary standards such as ISO 14000. In each issue, you"ll find in-depth articles and the most current case studies of successful environmental quality improvement efforts -- and guidance on how you can apply these goals to your organization. Written by leading industry experts and practitioners, Environmental Quality Management brings you innovative practices in Performance Measurement...Life-Cycle Assessments...Safety Management... Environmental Auditing...ISO 14000 Standards and Certification..."Green Accounting"...Environmental Communication...Sustainable Development Issues...Environmental Benchmarking...Global Environmental Law and Regulation.