Inula viscosa (L). Aiton leaves extract ameliorate arthritis by antioxidative and anti-inflammatory effects in formaldehyde-induced arthritis in mice

IF 4.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of ethnopharmacology Pub Date : 2024-11-22 DOI:10.1016/j.jep.2024.119154

Sara Ouari , Nadia Benzidane , Mohamed Sofiane Merakeb , Chahla Bencharif , Lekhmici Arrar , Noureddine Bribi

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引用次数: 0

Abstract

Ethnopharmacological relevance

Inula viscosa (L.) Aiton is a traditional medicinal plant widely distributed and used in Mediterranean countries, its leaves are prepared by maceration to treat, rheumatic pain, inflammatory diseases, diabetes, anemia and cancer.

Aim of the study

The present study purpose to investigate the anti-inflammatory efficacy of I. viscosa leaves methanol extract (IVME) at three different doses on formaldehyde-induced arthritis in NMRI albinos mice.

Materials and methods

Mice were divided into six groups (n = 6) as follows: normal control, disease control, Diclofenac group (10 mg/kg, p.o. daily) and three groups, daily treated with 50, 100 and 200 mg/kg IVME (p.o.); Formaldehyde models were obtained by a sub-plantar administration of 20 μl of formaldehyde (3.75% v/v) into the right hind paws of NMRI albino mice on 1st and 3rd days of the 10 experimental days. Joint diameter was measured, arthritis severity was evaluated by inhibition of paw edema, histological changes, synovial hyperplasia and immune cells infiltration was evaluated by histological and immunohistochemical analyses of CD3⁺, CD20⁺ and CD68⁺. Post-mitochondrial supernatants (PMS) from liver tissues homogenates were collected for the assessment of enzymatic and non-enzymatic antioxidants: Catalase (CAT) & Myeloperoxidase (MPO) activities, glutathione (GSH) and an oxidative stress biomarker (nitric-oxide (NO)) level.

Results

Administration of I. visocsa (at low dose: 50 mg/kg) significantly (∗∗∗p < 0.001) ameliorated the induced arthritis severity, reduced hyperplasia of synovial membrane, bone erosion and immune cells infiltration (∗p < 0.05), resulted by restoration of paw diameter. It also decreased levels of NO (∗∗∗p < 0.001) and MPO activity (∗∗∗p < 0.001), and significantly restored GSH levels (∗p < 0.05) and CAT activity (∗∗∗p < 0.001) in liver tissues.

Conclusion

These findings suggest that I. viscosa leaves have an anti-arthritic property. Which is due to the combination of antioxidant activity regulating oxidative stress and anti-inflammatory effect by probably cytokines regulation.

Abstract Image

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粘鼠（Inula viscosa (L)）。艾通叶提取物通过抗氧化和抗炎作用改善甲醛诱导的小鼠关节炎。

民族药理学意义：粘鼠（Inula viscosa (L.) Aiton）是一种传统药用植物，在地中海国家广泛分布和使用，其叶子通过浸泡制备，可治疗风湿痛、炎症性疾病、糖尿病、贫血和癌症：本研究旨在探讨三种不同剂量的 I. viscosa 叶甲醇提取物（IVME）对 NMRI 白化小鼠甲醛诱导的关节炎的抗炎功效：小鼠分为以下六组（n=6）：正常对照组、疾病对照组、双氯芬酸组（10 毫克/千克，每天点滴）和每天用 50、100 和 200 毫克/千克 IVME（点滴）治疗的三组；在 10 个实验日的第 1 天和第 3 天，向 NMRI 白化小鼠的右后爪皮下注射 20 微升甲醛（3.75% v/v），以获得甲醛模型。测量关节直径，通过抑制爪水肿、组织学变化、滑膜增生和免疫细胞浸润来评估关节炎的严重程度，组织学和免疫组化分析包括 CD3+、CD20+ 和 CD68+。从肝组织匀浆中收集线粒体后上清液（PMS），用于评估酶和非酶抗氧化剂：过氧化氢酶（CAT）和髓过氧化物酶（MPO）活性、谷胱甘肽（GSH）和氧化应激生物标志物（一氧化氮（NO））水平：结果：服用 I. viscocsa（低剂量：50 毫克/千克）可显著（***p这些研究结果表明，粘鼠李叶具有抗关节炎的特性。这是由于抗氧化活性调节氧化应激和细胞因子调节抗炎作用的结合。

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.