tRNA-derived RNAs in human milk extracellular vesicles and associations with breastfeeding variables and maternal diet.

IF 3 4区医学 Q2 GENETICS & HEREDITY

Epigenomics Pub Date : 2024-12-01 Epub Date: 2024-11-24 DOI:10.1080/17501911.2024.2430943

Abigail Gaylord, Elizabeth A Holzhausen, Bridget Chalifour, William B Patterson, Pei Wen Tung, Andrea A Baccarelli, Michael I Goran, Tanya L Alderete, Allison Kupsco

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引用次数: 0

Abstract

Aims: To describe tDRs in human milk EVs and their associations with maternal body mass index, age, dietary indices, breastfeeding frequency, season and time of milk collection in a Latina population.

Materials & methods: We sequenced small RNAs from EVs from 109 mature human milk samples collected at 1 month after delivery in the Southern California Mother's Milk Study. We grouped tDRs using hierarchical clustering and clusters were compared across tDR characteristics. We analyzed associations of tDRs with intrinsic maternal variables (body mass index, age), maternal nutrition (caloric intake, Healthy Eating Index, Dietary Inflammatory Index), and variables related to feeding and milk collection (breastfeeding frequency, season and time of milk collection) using negative binomial models.

Results: We identified 338 tDRs expressed in 90% or more of milk EV samples, of which 113 were identified in all samples. tDR-1:26-Gly-CCC-1-M4 accounted for most reads (79%). Pathway analysis revealed a wide array of biological processes and disease mechanisms across the four tDR clusters. tDRs were associated with season of collection, time of collection, breastfeeding frequency, and the dietary inflammatory index.

Conclusions: tDRs are abundant in milk EVs and may be sensitive to maternal diet, seasonality, time of day, and breastfeeding frequency.

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人乳细胞外囊泡中的 tRNA 衍生 RNA 及其与母乳喂养变量和母体饮食的关系。

目的：在拉丁裔人群中描述母乳 EV 中的 tDRs 及其与母亲体重指数、年龄、饮食指数、母乳喂养频率、季节和采奶时间的关系：我们对南加州母乳研究（Southern California Mother's Milk Study）中产后 1 个月采集的 109 份成熟母乳样本中的 EVs 小 RNA 进行了测序。我们使用分层聚类对 tDR 进行了分组，并对不同 tDR 特征的聚类进行了比较。我们使用负二项模型分析了 tDR 与母体内在变量（体重指数、年龄）、母体营养（热量摄入、健康饮食指数、膳食炎症指数）以及喂养和采奶相关变量（母乳喂养频率、采奶季节和时间）的关系：我们在90%或更多的牛奶EV样本中发现了338个tDR，其中113个在所有样本中都被发现。tDR与采集季节、采集时间、母乳喂养频率和饮食炎症指数有关。结论：tDR在牛奶EV中含量丰富，可能对母体饮食、季节性、时间和母乳喂养频率很敏感。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Epigenomics GENETICS & HEREDITY-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

>12 weeks

期刊介绍： Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.