Isolation, identification, and characterization of Bacillus subtilis SMP-2 from panitenga and exploring its potential for biosurfactant production

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2024-11-19 DOI:10.1016/j.fbp.2024.11.018

Smrity Sonbhadra, Lalit M. Pandey

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引用次数: 0

Abstract

A traditional Assamese fermented food, Panitenga, was explored for potential probiotic isolation. A potent bacterial strain exhibiting biosurfactant production ability was isolated, characterized, and identified as Bacillus subtilis SMP-2. Environmental parameters were optimized for maximum cell growth and biosurfactant production that was found to be pH 6, 30°C, 130 rpm, with glycerol as the carbon (C) source, yeast extract as the nitrogen (N) source, and a C/N ratio of 3:1. At these optimized conditions, the strain yielded 8.13 ± 0.9 g/L of lipopeptide biosurfactant. This biosurfactant remarkably reduced the surface tension of water from 72 mN/m to 37 ± 0.36 mN/m, possessed a low critical micelle concentration (CMC) of 125 mg/L, and exhibited antimicrobial properties. It persisted to be stable across a wide range of physical conditions, including variations in pH, temperature, and salinity. The significantly high production yield and noteworthy properties of the biosurfactant from Bacillus subtilis SMP-2 make it a promising candidate for applications in microbial-enhanced oil recovery (MEOR), bioremediation of oil spills, production of cosmetics and pharmaceuticals, contributing to economic growth and sustainable development.

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从 Panitenga 分离、鉴定和表征枯草芽孢杆菌 SMP-2 并探索其生产生物表面活性剂的潜力

研究人员对一种传统的阿萨姆发酵食品 Panitenga 进行了潜在益生菌分离研究。分离、鉴定并确定了一种具有生产生物表面活性剂能力的强效细菌菌株，即枯草芽孢杆菌 SMP-2。为实现最大的细胞生长和生物表面活性剂产量，对环境参数进行了优化，发现 pH 值为 6，温度为 30°C，转速为 130 rpm，碳（C）源为甘油，氮（N）源为酵母提取物，C/N 比为 3:1。在这些优化条件下，该菌株产生了 8.13 ± 0.9 克/升的脂肽生物表面活性剂。这种生物表面活性剂显著降低了水的表面张力，从 72 mN/m 降至 37 ± 0.36 mN/m，临界胶束浓度（CMC）低至 125 mg/L，并具有抗菌特性。它在各种物理条件下（包括 pH 值、温度和盐度变化）都能保持稳定。从枯草芽孢杆菌 SMP-2 中提取的生物表面活性剂产量很高，而且具有显著的特性，因此有望应用于微生物强化采油（MEOR）、溢油的生物修复、化妆品和药品的生产，从而促进经济增长和可持续发展。

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.