Development of a sustainable extraction and storage stability of antioxidant and anticholinergic pressurized natural deep eutectic solvent extracts from Citrus reticulata leaves

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2024-11-08 DOI:10.1016/j.fbp.2024.11.006

Gloria Domínguez-Rodríguez , Victor M. Amador-Luna , José A. Mendiola , Fabián Parada-Alfonso , Elena Ibáñez

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引用次数: 0

Abstract

The recovery of phenolic compounds from C. reticulata leaves using pressurized liquid extraction (PLE) combined with Natural Deep Eutectic Solvents (NaDES) employing Choline chloride:Glycerol (1:2 molar ratio) as NaDES was investigated for the first time. A Box-Behnken experimental design was carried out to observe the influence of time, temperature and % water in NaDES on the extraction of compounds with antioxidant and anticholinergic (AC) capacities and only with antioxidant capacity (A). Besides, optimum extracts were compared with PLE extracts obtained under the same extraction conditions but using ethanol-/water (70:30, v/v). PLE-NaDES-AC extract obtained at 150°C during 25 min with 57.9 % water in NaDES displayed the highest in vitro antioxidant and anticholinergic capacities and the highest number of different phenolic compounds identified by HPLC-IT-MS compared with the rest of extracts. Lower extraction temperature (64°C) was necessary for obtaining extracts with only antioxidant capacity. Besides, a forced stability study showed a high protection effect of NaDES on the degradation of antioxidant phenolic compounds compared to ethanolic extracts. Results indicated that the use of PLE-NaDES is a sustainable alternative to ethanolic PLE extraction for the recovery of stable bioactive phenolic compounds.

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从柑橘网纹叶中提取抗氧化剂和抗胆碱能加压天然深共晶溶剂提取物的可持续提取和储存稳定性的开发

研究人员首次采用氯化胆碱：甘油（摩尔比为 1:2）作为 NaDES，结合天然深层共晶溶剂（NaDES）进行加压液体萃取（PLE），从网纹叶中回收酚类化合物。采用 Box-Behnken 实验设计，观察 NaDES 中的时间、温度和水的百分比对抗氧化能力和抗胆碱能（AC）以及仅抗氧化能力（A）的化合物萃取的影响。此外，还将最佳提取物与在相同提取条件下使用乙醇/水（70:30，v/v）提取的 PLE 提取物进行了比较。与其他提取物相比，PLE-NaDES-AC 提取物的体外抗氧化和抗胆碱能能力最高，HPLC-IT-MS 鉴定出的不同酚类化合物数量也最多。要获得仅具有抗氧化能力的提取物，必须降低提取温度（64°C）。此外，强制稳定性研究表明，与乙醇提取物相比，NaDES 对抗氧化酚类化合物的降解具有很高的保护作用。结果表明，在回收稳定的生物活性酚类化合物方面，使用 PLE-NaDES 是乙醇提取 PLE 的一种可持续替代方法。

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.