Whole-Genome sequencing of Calonectria dianii: An important pathogen causing Eucalyptus leaf blight

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2024-11-01 DOI:10.1016/j.ygeno.2024.110967

QianLi Liu , FeiFei Liu , GuoQing Li , KaiDong Liu , ZhenChi Huang , ShanNi Cao , JunDi Zhong

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引用次数: 0

Abstract

Eucalyptus leaf blight, caused by Calonectria spp., significantly impacts the global Eucalyptus industry. Calonectria dianii, as one of the predominant causal agents, poses a serious threat to Eucalyptus plantations in China. To enhance our understanding of its pathogenic mechanisms, we sequenced the genome of C. dianii RIFT 6520 using both Nanopore PromethION and Illumina NovaSeq PE150 platforms. Our analysis revealed a 61.76 Mb genome comprising 30 contigs with an N50 of 4,726,631 bp, a GC content of 49.74 %, and 10,184 predicted coding genes. Additionally, comparative genomic analysis between C. dianii and seven other significant plant-pathogenic Calonectria species was conducted. This analysis provided insights into the evolutionary relationships and adaptive mechanisms of these pathogens. Our study elucidates the genetic basis of C. dianii's pathogenicity and evolution, providing valuable information for future research on its molecular interactions with Eucalyptus and aiding in the development of precise control measures for Eucalyptus leaf blight.

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Calonectria dianii 的全基因组测序：引起桉树叶枯病的一种重要病原体。

Calonectria spp.引起的桉树叶枯病严重影响着全球桉树产业。Calonectria dianii 是主要病原菌之一，对中国的桉树种植园构成严重威胁。为了加深对其致病机制的了解，我们使用 Nanopore PromethION 和 Illumina NovaSeq PE150 平台对 C. dianii RIFT 6520 的基因组进行了测序。我们的分析结果显示，基因组大小为 61.76 Mb，由 30 个等位基因组成，N50 为 4,726,631 bp，GC 含量为 49.74 %，预测编码基因为 10,184 个。此外，还对 C. dianii 和其他 7 个重要的植物致病性 Calonectria 物种进行了基因组比较分析。这项分析有助于深入了解这些病原体的进化关系和适应机制。我们的研究阐明了 C. dianii 的致病性和进化的遗传基础，为今后研究其与桉树的分子相互作用提供了宝贵的信息，并有助于制定精确的桉树叶枯病控制措施。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.