Application of UPLC-MS/MS to Study Cellular Pharmacokinetics of Seven Active Components of Cnidii Fructus Extracts.

IF 2.1 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current drug metabolism Pub Date : 2024-11-18 DOI:10.2174/0113892002301262241107065717

Yu Bai, Huizi Ouyang, Yang Liu, Fanjiao Zuo, Caixia Li, Shuting Zhou, Yanxu Chang, Jun He

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引用次数: 0

Abstract

Background: Cnidii Fructus (CF) is a herbal medicine with pharmacological activities such as antitumor, antiviral, antiallergic, antipruritic effects, and so on.

Objective: In this study, an ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC- MS/MS) method was prepared and verified to measure the concentrations of seven analytes (bergapten, xanthotoxol, xanthotoxin, imperatorin, osthole, isopimpinellin, isoimperatorin) in HepG2 cells.

Methods: The separation of seven analytes was performed on an ACQUITY UPLC® BEH C18 column (2.1×100 mm, 1.7 μm) with a gradient mobile phase system of 0.1% formic acid/water and acetonitrile.

Results: The CV of analytes was within 7.77%, and the bias was in the range of -5.43%-3.84%. The matrix effects of analytes ranged from 92.95% to 104.58%, and the extraction recoveries ranged from 76.45% to 104.69%. The relative standard deviation of stability results was less than 8.21%, indicating that seven analytes were stable.

Conclusion: The method was successfully applied to the determination of the content of seven analytes of CF extracts by UPLC-MS/MS, and the results will provide a reference for the cellular pharmacokinetics of CF.

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应用 UPLC-MS/MS 研究蛇床子提取物中七种活性成分的细胞药代动力学

背景：蛇床子是一种中药材，具有抗肿瘤、抗病毒、抗过敏、止痒等药理作用：本研究制备并验证了超高效液相色谱/串联质谱（UPLC- MS/MS）方法，用于测定HepG2细胞中7种分析物（小檗苷、黄毒酚、黄毒苷、欧蛇床子苷、异欧蛇床子苷、异欧蛇床子苷）的浓度：采用ACQUITY UPLC® BEH C18色谱柱（2.1×100 mm，1.7 μm），以0.1%甲酸/水和乙腈为流动相进行梯度洗脱，分离了7种分析物：分析物的检出限（CV）在 7.77%以内，偏差在-5.43%-3.84%之间。分析物的基质效应为 92.95% 至 104.58%，萃取回收率为 76.45% 至 104.69%。稳定性结果的相对标准偏差小于8.21%，表明7种分析物稳定：该方法成功地应用于UPLC-MS/MS测定CF提取物中7种分析物的含量，其结果将为CF的细胞药代动力学提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current drug metabolism 医学-生化与分子生物学

CiteScore

4.30

自引率

4.30%

发文量

审稿时长

4-8 weeks

期刊介绍： Current Drug Metabolism aims to cover all the latest and outstanding developments in drug metabolism, pharmacokinetics, and drug disposition. The journal serves as an international forum for the publication of full-length/mini review, research articles and guest edited issues in drug metabolism. Current Drug Metabolism is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the most important developments. The journal covers the following general topic areas: pharmaceutics, pharmacokinetics, toxicology, and most importantly drug metabolism. More specifically, in vitro and in vivo drug metabolism of phase I and phase II enzymes or metabolic pathways; drug-drug interactions and enzyme kinetics; pharmacokinetics, pharmacokinetic-pharmacodynamic modeling, and toxicokinetics; interspecies differences in metabolism or pharmacokinetics, species scaling and extrapolations; drug transporters; target organ toxicity and interindividual variability in drug exposure-response; extrahepatic metabolism; bioactivation, reactive metabolites, and developments for the identification of drug metabolites. Preclinical and clinical reviews describing the drug metabolism and pharmacokinetics of marketed drugs or drug classes.