Regeneration of Spent Culture Media for Sustainable and Continuous mAb Production via Ion Concentration Polarization.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2024-11-18 DOI:10.1002/bit.28888

Eric Wynne, Junghyo Yoon, Dohyun Park, Mingyang Cui, Caitlin Morris, Jaeweon Lee, Zhao Wang, Seongkyu Yoon, Jongyoon Han

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引用次数: 0

Abstract

In modern bioprocessing, cell culture media is one of the most significant cost drivers, yet the nutrients and other critical factors in the media are often not fully utilized. With the renewed emphasis on reducing the cost of bioprocessing, there is much interest in reducing the overall use of cell culture media. In this work, we introduce a mesoscale microfluidic separation device based on the ion concentration polarization (ICP) process to regenerate the spent media for reuse by removing critical waste products from the cell culture that are known to inhibit the growth of the cells. We demonstrated that up to 75% of spent culture media can be regenerated and reused without affecting the cell viability. A detailed analysis of the materials consumed during antibody production indicated that one could improve the water process mass intensity by up to 33% by regenerating and recycling the media. Given that ICP separation systems have already been scaled up to support large-volume processing, it would be feasible to deploy this technology for manufacturing scale bioreactors (e.g., 50 L perfusion culture of CHO cells), reducing the overall operation cost and water use.

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通过离子浓度极化再生废培养基，实现可持续和连续的 mAb 生产。

在现代生物处理过程中，细胞培养基是最重要的成本驱动因素之一，但培养基中的营养物质和其他关键因素往往没有得到充分利用。随着人们对降低生物处理成本的重新重视，减少细胞培养基的总体使用量备受关注。在这项工作中，我们引入了一种基于离子浓度极化（ICP）工艺的中尺度微流体分离装置，通过去除细胞培养物中已知会抑制细胞生长的关键废品，再生废培养基以供再利用。我们证明，多达 75% 的废培养基可以再生和重复使用，而不会影响细胞活力。对抗体生产过程中所消耗材料的详细分析表明，通过再生和循环利用培养基，可以将水处理过程的质量强度提高 33%。鉴于 ICP 分离系统已经扩大到支持大容量处理，因此将这项技术应用于生产规模的生物反应器（如 50 升 CHO 细胞灌流培养）是可行的，从而降低了整体运营成本和用水量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

期刊介绍： Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.