The Expressions of p16, HPV16-L1 and HPV18-E6 in Salivary Gland Tumours.

IF 0.6 Q4 SURGERY

Indian Journal of Otolaryngology and Head and Neck Surgery Pub Date : 2024-12-01 Epub Date: 2024-09-11 DOI:10.1007/s12070-024-05007-w

Nurul Najwa Mohd Zakir, Norhafiza Mat Lazim, Anani Aila Mat Zin, Ali Haron, Baharudin Abdullah

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引用次数: 0

Abstract

Aims: This study aimed to investigate the relationship between human papillomavirus (HPV) infection and the development of salivary gland tumour (SGT) by evaluating the expression of p16 and the oncoproteins HPV16-L1 and HPV18-E6 using immunohistochemical (IHC) staining. Additionally, we assess the agreement between these oncoproteins and p16 in diagnosing HPV-infected SGT.

Methods: This cross-sectional study included all SGT cases undergoing surgical resection at Hospital Universiti Sains Malaysia (HUSM) and Hospital Raja Perempuan Zainab II (HRPZ) in Malaysia from April 2022 to April 2023. IHC staining was performed on paraffin-embedded tissues at the Pathology Laboratory, HUSM, to evaluate the expression of p16, HPV16-L1, and HPV18-E6 oncoproteins. The clinicopathological data were correlated with the staining results.

Results: 49 SGT cases were identified, mainly in middle-aged Malay women, with most tumours originating from the parotid gland. Malignant tumours included mucoepidermoid carcinoma (22.4%), adenoid cystic carcinoma (4.1%), acinic cell carcinoma (4.1%), and adenocarcinoma (2%). Benign tumours primarily consisted of pleomorphic adenoma (49%) and Warthin tumours (16.3%). Positive p16 expression was detected in 67% of cases, while HPV16 and HPV18 were detected in 65% and 90% of tumours, respectively. HPV16-L1 exhibited 75.8% sensitivity and 56.3% specificity, while HPV18-E6 showed 100% sensitivity and 31.2% specificity compared to p16.

Conclusion: The study findings suggest a correlation between the presence of high-risk HPV types 16 and 18 and the development of SGT, as evidenced by the overexpression of p16, HPV16-L1, and HPV18-E6 oncoproteins. Both HPV16-L1 and HPV18-E6 tests are acceptable, reliable, and sensitive for detecting high-risk HPV in SGT.

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唾液腺肿瘤中 p16、HPV16-L1 和 HPV18-E6 的表达。

目的：本研究旨在通过使用免疫组织化学（IHC）染色评估 p16 和肿瘤蛋白 HPV16-L1 及 HPV18-E6 的表达，研究人类乳头瘤病毒（HPV）感染与唾液腺肿瘤（SGT）发病之间的关系。此外，我们还评估了这些肿瘤蛋白与 p16 在诊断受 HPV 感染的 SGT 方面的一致性：这项横断面研究包括2022年4月至2023年4月期间在马来西亚森斯大学医院（HUSM）和拉贾培仁布医院（HRPZ II）接受手术切除的所有SGT病例。HUSM 病理实验室对石蜡包埋组织进行了 IHC 染色，以评估 p16、HPV16-L1 和 HPV18-E6 肿瘤蛋白的表达。临床病理数据与染色结果相关：结果：共发现 49 例 SGT 病例，主要发生在中年马来女性身上，大多数肿瘤来自腮腺。恶性肿瘤包括粘液表皮样癌（22.4%）、腺样囊性癌（4.1%）、尖细胞癌（4.1%）和腺癌（2%）。良性肿瘤主要包括多形性腺瘤（49%）和Warthin瘤（16.3%）。在 67% 的病例中检测到 p16 阳性表达，而在 65% 和 90% 的肿瘤中分别检测到 HPV16 和 HPV18。与 p16 相比，HPV16-L1 的敏感性为 75.8%，特异性为 56.3%，而 HPV18-E6 的敏感性为 100%，特异性为 31.2%：研究结果表明，p16、HPV16-L1 和 HPV18-E6 肿瘤蛋白的过度表达表明，高危型 HPV16 和 18 与 SGT 的发生存在相关性。HPV16-L1 和 HPV18-E6 检测对检测 SGT 中的高危型 HPV 都是可接受的、可靠的和敏感的。

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来源期刊

Indian Journal of Otolaryngology and Head and Neck Surgery Medicine-Otorhinolaryngology

CiteScore

0.80

自引率

0.00%

发文量

226

审稿时长

6-12 weeks

期刊介绍： Indian Journal of Otolaryngology and Head & Neck Surgery was founded as Indian Journal of Otolaryngology in 1949 as a scientific Journal published by the Association of Otolaryngologists of India and was later rechristened as IJOHNS to incorporate the changes and progress. IJOHNS, undoubtedly one of the oldest Journals in India, is the official publication of the Association of Otolaryngologists of India and is about to publish it is 67th Volume in 2015. The Journal published quarterly accepts articles in general Oto-Rhino-Laryngology and various subspecialities such as Otology, Rhinology, Laryngology and Phonosurgery, Neurotology, Head and Neck Surgery etc. The Journal acts as a window to showcase and project the clinical and research work done by Otolaryngologists community in India and around the world. It is a continued source of useful clinical information with peer review by eminent Otolaryngologists of repute in their respective fields. The Journal accepts articles pertaining to clinical reports, Clinical studies, Research articles in basic and applied Otolaryngology, short Communications, Clinical records reporting unusual presentations or lesions and new surgical techniques. The journal acts as a catalyst and mirrors the Indian Otolaryngologist’s active interests and pursuits. The Journal also invites articles from senior and experienced authors on interesting topics in Otolaryngology and allied sciences from all over the world. The print version is distributed free to about 4000 members of Association of Otolaryngologists of India and the e-Journal shortly going to make its appearance on the Springer Board can be accessed by all the members. Association of Otolaryngologists of India and M/s Springer India group have come together to co-publish IJOHNS from January 2007 and this bondage is going to provide an impetus to the Journal in terms of international presence and global exposure.