Dose-specific toxicity of naphthalene (Polycyclic Aromatic Hydrocarbon) and subsequent recovery by humic feed-o-fert on Anabas testudineus (Bloch): Blood biochemical and morphological introspections

IF 2.1 4区环境科学与生态学 Q3 ECOLOGY

Regional Studies in Marine Science Pub Date : 2024-11-08 DOI:10.1016/j.rsma.2024.103912

Sukhendu Dey , Megha Santra , Subhas Das, Apurba Ratan Ghosh

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引用次数: 0

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are pervasive contaminants in aquatic ecosystems, yet their specific effects on blood pathophysiology, including alterations in blood biochemical and morphological introspections, remain largely unexplored. As consequence, the present study is aimed to record the toxicity of naphthalene exposure for 21-d under two selected doses 0.7075 mg/L (T1) and 1.415 mg/L (T2), i.e., 25 and 50 % of LC₅₀ value [2.83 mg/L] respectively and subsequent recovery with one improved organic solid waste, feed-promoter Humic feed-o-fert (HF) of 0.5 g/L for the same duration of 21-d. The results showed significant increment in values in blood biochemical parameters, viz., cholesterol (CHOLES), glucose (GLU), glutamine pyruvic transmission (GPT), calcium (Ca), alkaline phosphatase (ALP), high density lipoprotein (HDL) and low density lipoprotein (LDL), and some displayed decreasing trend like protein (PRO), albumin (ALB) and triglyceride (Trig) under this exposure. During recovery phase with HF, the nature of biochemical changes became reversed showing significant restoration and repairment in comparison to naphthalene treatment. Morphological alterations of blood cells revealed maximum changes and appearance, such as tear drop-like cells (Tr), sickle cells (Sk), swelled cells (Sc) and vacuolated cells (Va) under two doses of exposure in 21-d, but under recovery with HF the cells approached towards regaining the normal contour of leucocytes (L), erythrocytes (Er) and tear drop like cells (Tr). So, it can be inferred that Humic feed-o-fert (HF) here played an important role to resist the haematological impairments in fish against the naphthalene (PAH) exposure. Further, Humic feed-o-fert (HF) may contribute in aquatic ecosystem restoration against the environmental contaminants, especially PAHs in aquaculture.

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萘（多环芳香烃）的剂量特异性毒性以及腐殖质饲料-肥料对 Anabas testudineus (Bloch) 的后续恢复作用：血液生化和形态学观察

多环芳烃（PAHs）是水生生态系统中普遍存在的污染物，但其对血液病理生理学的具体影响，包括血液生化和形态学方面的改变，在很大程度上仍未得到研究。因此，本研究旨在记录萘在两种选定剂量 0.7075 毫克/升（T1）和 1.415 毫克/升（T2）（即分别为半数致死浓度[2.83 毫克/升]的 25% 和 50%）下暴露 21 天的毒性，以及随后使用一种改良的有机固体废物--0.5 克/升的饲料促进剂腐植酸饲料-奥肥料（HF）--进行同样持续 21 天的恢复。结果表明，血液生化指标，即胆固醇（CHOLES）、葡萄糖（GLU）、谷氨酰胺丙酮酸传递（GPT）、钙（Ca）、碱性磷酸酶（ALP）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）的值明显增加，而一些指标则呈下降趋势，如蛋白质（PRO）、白蛋白（ALB）和甘油三酯（Trig）。在高频炉的恢复阶段，生化变化的性质发生了逆转，与萘处理相比，出现了明显的恢复和修复。在 21 天的两种剂量接触中，血细胞的形态变化最大，如泪滴样细胞（Tr）、镰刀形细胞（Sk）、肿胀细胞（Sc）和空泡细胞（Va），但在使用 HF 的恢复期，细胞恢复了正常轮廓，如白细胞（L）、红细胞（Er）和泪滴样细胞（Tr）。因此可以推断，腐植酸饲料-肥料（HF）在防止鱼类因接触萘（PAH）而出现血液损伤方面发挥了重要作用。此外，腐植酸饲料-奥-肥料（HF）可能有助于恢复水生生态系统，抵御环境污染物，尤其是水产养殖中的多环芳烃。

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来源期刊

Regional Studies in Marine Science Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

3.90

自引率

4.80%

发文量

336

审稿时长

69 days

期刊介绍： REGIONAL STUDIES IN MARINE SCIENCE will publish scientifically sound papers on regional aspects of maritime and marine resources in estuaries, coastal zones, continental shelf, the seas and oceans.