Green synthesis of silver nanoparticle by Hyoscyamus muticus L. extract and study of its effect on tomato infected with Meloidogyne javanica

IF 4 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Structure Pub Date : 2024-11-05 DOI:10.1016/j.molstruc.2024.140605

Seyyed Maryam Mousavi Kordsholie , Hamid Reza Rajabi , Habiballah Chadegani

{"title":"Green synthesis of silver nanoparticle by Hyoscyamus muticus L. extract and study of its effect on tomato infected with Meloidogyne javanica","authors":"Seyyed Maryam Mousavi Kordsholie , Hamid Reza Rajabi , Habiballah Chadegani","doi":"10.1016/j.molstruc.2024.140605","DOIUrl":null,"url":null,"abstract":"<div><div>In this study, the aqueous leaf extract of <em>Hyoscyamus muticus L</em> Plant leaf was prepared using three methods: maceration (MAC), microwave-assisted extraction (MAE), and ultrasonic-assisted extraction (UAE). Then, the extracts were used to synthesize silver nanoparticles (Ag NPs) in an environmentally friendly approach. The characterization of the synthesized Ag NPs was carried out by various techniques. The XRD results confirmed the successful synthesis of nano-sized Ag crystals with spherical structure. The SEM images showed the formation of Ag NPs, which were smaller than 75 nm. The UV–Vis studies showed an obvious absorption peak for Ag NPs between 400 and 500 nm, with a clear peak around 450 nm for all samples. The elemental analysis using the EDS technique confirmed the presence of silver in the synthesized Ag NPs. Furthermore, the effect of different concentrations of Ag NPs was investigated on hatching and second-stage juvenile (J2s) mortality of <em>Meloidogyne javanica</em>, under controlled conditions. The results showed that increasing concentrations of Ag NPs led to higher percentages of inhibition of egg hatching and J2s mortality. Additionally, the Ag NPs synthesized by ultrasonic-assisted extraction with lower lethal concentration were found to be more toxic to <em>M. javanica</em> than the extract prepared by soaking. According to the results, Ag NPs showed a stronger inhibitory effect on <em>M. javanica</em> egg hatching and J2s mortality compared to the total extracts and chemically synthesized Ag NPs. Also, the lowest LC50 was observed for the Ag NPs synthesized using the plant extract as: UAE > MAE (180 w) > MAE (90 w) > MAC methods. The highest inhibition of egg hatch occurred at 0.5% v/v of Ag NPs after 72 h and remained consistent after 120 h.</div></div>","PeriodicalId":16414,"journal":{"name":"Journal of Molecular Structure","volume":"1323 ","pages":"Article 140605"},"PeriodicalIF":4.0000,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Structure","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022286024031132","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, the aqueous leaf extract of Hyoscyamus muticus L Plant leaf was prepared using three methods: maceration (MAC), microwave-assisted extraction (MAE), and ultrasonic-assisted extraction (UAE). Then, the extracts were used to synthesize silver nanoparticles (Ag NPs) in an environmentally friendly approach. The characterization of the synthesized Ag NPs was carried out by various techniques. The XRD results confirmed the successful synthesis of nano-sized Ag crystals with spherical structure. The SEM images showed the formation of Ag NPs, which were smaller than 75 nm. The UV–Vis studies showed an obvious absorption peak for Ag NPs between 400 and 500 nm, with a clear peak around 450 nm for all samples. The elemental analysis using the EDS technique confirmed the presence of silver in the synthesized Ag NPs. Furthermore, the effect of different concentrations of Ag NPs was investigated on hatching and second-stage juvenile (J2s) mortality of Meloidogyne javanica, under controlled conditions. The results showed that increasing concentrations of Ag NPs led to higher percentages of inhibition of egg hatching and J2s mortality. Additionally, the Ag NPs synthesized by ultrasonic-assisted extraction with lower lethal concentration were found to be more toxic to M. javanica than the extract prepared by soaking. According to the results, Ag NPs showed a stronger inhibitory effect on M. javanica egg hatching and J2s mortality compared to the total extracts and chemically synthesized Ag NPs. Also, the lowest LC50 was observed for the Ag NPs synthesized using the plant extract as: UAE > MAE (180 w) > MAE (90 w) > MAC methods. The highest inhibition of egg hatch occurred at 0.5% v/v of Ag NPs after 72 h and remained consistent after 120 h.

Abstract Image

查看原文本刊更多论文

用 Hyoscyamus muticus L. 提取物绿色合成银纳米粒子并研究其对感染 Meloidogyne javanica 的番茄的影响

本研究采用浸渍法（MAC）、微波辅助萃取法（MAE）和超声辅助萃取法（UAE）三种方法制备了植物叶 Hyoscyamus muticus L 的水性叶提取物。然后，利用提取物以环境友好的方法合成银纳米粒子（Ag NPs）。合成的银纳米粒子采用了多种技术进行表征。XRD 结果证实成功合成了具有球形结构的纳米级银晶体。扫描电镜图像显示形成了小于 75 纳米的 Ag NPs。紫外可见光研究表明，Ag NPs 在 400 至 500 纳米之间有一个明显的吸收峰，所有样品在 450 纳米附近都有一个清晰的吸收峰。利用 EDS 技术进行的元素分析证实了合成的 Ag NPs 中含有银。此外，在受控条件下，研究了不同浓度的银纳米粒子对爪螨孵化和第二阶段幼虫（J2s）死亡率的影响。结果表明，Ag NPs 浓度越高，对卵孵化和 J2s 死亡率的抑制率越高。此外，与浸泡提取物相比，通过超声波辅助提取合成的致死浓度较低的银氧化物对爪螨的毒性更大。结果表明，与总提取物和化学合成的 Ag NPs 相比，Ag NPs 对爪哇蝇卵孵化和 J2s 死亡率有更强的抑制作用。此外，使用植物提取物合成的 Ag NPs LC50 最低：UAE > MAE (180 w) > MAE (90 w) > MAC 方法。72 小时后，0.5% v/v 的 Ag NPs 对卵孵化的抑制率最高，120 小时后仍保持不变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Molecular Structure 化学-物理化学

CiteScore

7.10

自引率

15.80%

发文量

2384

审稿时长

45 days

期刊介绍： The Journal of Molecular Structure is dedicated to the publication of full-length articles and review papers, providing important new structural information on all types of chemical species including: • Stable and unstable molecules in all types of environments (vapour, molecular beam, liquid, solution, liquid crystal, solid state, matrix-isolated, surface-absorbed etc.) • Chemical intermediates • Molecules in excited states • Biological molecules • Polymers. The methods used may include any combination of spectroscopic and non-spectroscopic techniques, for example: • Infrared spectroscopy (mid, far, near) • Raman spectroscopy and non-linear Raman methods (CARS, etc.) • Electronic absorption spectroscopy • Optical rotatory dispersion and circular dichroism • Fluorescence and phosphorescence techniques • Electron spectroscopies (PES, XPS), EXAFS, etc. • Microwave spectroscopy • Electron diffraction • NMR and ESR spectroscopies • Mössbauer spectroscopy • X-ray crystallography • Charge Density Analyses • Computational Studies (supplementing experimental methods) We encourage publications combining theoretical and experimental approaches. The structural insights gained by the studies should be correlated with the properties, activity and/ or reactivity of the molecule under investigation and the relevance of this molecule and its implications should be discussed.