Mesenchymal stem cell-derived protein extract induces periodontal regeneration

IF 3.7 3区医学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotherapy Pub Date : 2025-02-01 DOI:10.1016/j.jcyt.2024.10.003

Yihao Peng , Kengo Iwasaki , Yoichiro Taguchi , Isao Ishikawa , Makoto Umeda

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引用次数: 0

Abstract

Background

Periodontal disease is characterized by chronic inflammation and destruction of supporting periodontal tissues, ultimately leading to tooth loss. In recent years, “cell-free treatment” without stem cell transplantation has attracted considerable attention for tissue regeneration. This study investigated the effects of extracts of mesenchymal stem cells (MSC-extract) and their protein components (MSC-protein) on the proliferation and migration of periodontal ligament (PDL) cells and whether MSC-protein can induce periodontal regeneration.

Methods

MSC-extract and MSC-protein were obtained by subjecting mesenchymal stem cells (MSCs) to freeze–thaw cycles and acetone precipitation. Cell proliferation was examined using a WST-8 assay and Ki67 immunostaining, and cell migration was examined using Boyden chambers. The MSC-protein content was analyzed using liquid chromatography-mass spectrometry, protein arrays, and enzyme-linked immunosorbent assays (ELISAs). Gene expression in MSC-protein-treated PDL cells was examined using RNA-sequencing and Gene Ontology analyses. The regenerative potential of MSC-protein was examined using micro-computer tomography (CT) and histological analyses after transplantation into a rat periodontal defect model.

Results

MSC-extract and MSC-protein promoted the proliferation and migration of PDL cells. Protein array and ELISA revealed that MSC-protein contained high concentrations of basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Exogenous bFGF promoted the proliferation and migration of PDL cells. Furthermore, the transplantation of MSC-protein enhanced periodontal tissue regeneration with the formation of new alveolar bone and PDLs.

Conclusions

These results indicate that the MSC-protein promotes the proliferation and migration of PDL cells and induces significant periodontal tissue regeneration, suggesting that the MSC-protein could be used as a new cell-free treatment for periodontal disease.

Abstract Image

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间充质干细胞衍生蛋白提取物可诱导牙周再生。

背景：牙周病的特点是慢性炎症和牙周支持组织的破坏，最终导致牙齿脱落。近年来，无干细胞移植的 "无细胞治疗 "在组织再生方面引起了广泛关注。本研究探讨了间充质干细胞提取物（间充质干细胞提取物）及其蛋白成分（间充质干细胞蛋白）对牙周韧带（PDL）细胞增殖和迁移的影响，以及间充质干细胞蛋白能否诱导牙周再生：方法：间充质干细胞（MSCs）通过冻融循环和丙酮沉淀获得间充质干细胞提取物和间充质干细胞蛋白。细胞增殖采用WST-8检测法和Ki67免疫染色法，细胞迁移采用波登室法。使用液相色谱-质谱法、蛋白质阵列和酶联免疫吸附试验（ELISA）分析了间充质干细胞蛋白质的含量。使用 RNA 测序和基因本体分析法检测了间充质干细胞蛋白处理过的 PDL 细胞中的基因表达。将间叶干细胞蛋白移植到大鼠牙周缺损模型中后，利用微型计算机断层扫描（CT）和组织学分析检测了间叶干细胞蛋白的再生潜力：结果：间充质干细胞提取物和间充质干细胞蛋白促进了PDL细胞的增殖和迁移。蛋白质阵列和酶联免疫吸附试验表明，间充质干细胞蛋白含有高浓度的碱性成纤维细胞生长因子（bFGF）和肝细胞生长因子（HGF）。外源性bFGF能促进PDL细胞的增殖和迁移。此外，间充质干细胞蛋白的移植促进了牙周组织的再生，形成了新的牙槽骨和 PDL：这些结果表明，间充质干细胞蛋白能促进PDL细胞的增殖和迁移，并诱导牙周组织的显著再生，这表明间充质干细胞蛋白可作为一种新的无细胞治疗牙周疾病的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytotherapy 医学-生物工程与应用微生物

CiteScore

6.30

自引率

4.40%

发文量

683

审稿时长

49 days

期刊介绍： The journal brings readers the latest developments in the fast moving field of cellular therapy in man. This includes cell therapy for cancer, immune disorders, inherited diseases, tissue repair and regenerative medicine. The journal covers the science, translational development and treatment with variety of cell types including hematopoietic stem cells, immune cells (dendritic cells, NK, cells, T cells, antigen presenting cells) mesenchymal stromal cells, adipose cells, nerve, muscle, vascular and endothelial cells, and induced pluripotential stem cells. We also welcome manuscripts on subcellular derivatives such as exosomes. A specific focus is on translational research that brings cell therapy to the clinic. Cytotherapy publishes original papers, reviews, position papers editorials, commentaries and letters to the editor. We welcome "Protocols in Cytotherapy" bringing standard operating procedure for production specific cell types for clinical use within the reach of the readership.