Probiotic and functional characterization of newly isolated Lactiplantibacillus plantarum strains from human breast milk and proliferative inhibition potential of metabolites

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2024-11-07 DOI:10.1016/j.enzmictec.2024.110545

Yusuf Alan , Ali-Osman Keskin , Mehmet Sönmez

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引用次数: 0

Abstract

Four Lactiplantibacillus plantarum strains newly isolated and identified from human breast milk in Türkiye, have probiotic, functional and proliferative inhibition potential of metabolites against colon cancer cell lines were evaluated. In simulated gastric and intestinal media, all strains exhibited strong probiotic character by showing resistance, although decreasing with time and concentration. The strains were sensitive to penicillin G, rifampin and chloramphenicol and showed antibacterial effect on all pathogenic bacteria. Citric acid, malic acid, tartaric acid, pyruvic acid and fumaric acid were not detected in the strains, while the highest amount of acetic acid was detected. The quantitative-qualitative analysis and structural characterization of exopolysaccharide (EPS) was confirmed and it was determined that the strains synthesized similar amounts. Compared to standard antioxidants, the strains showed less DPPH activity and similar ABTS activity. High amounts of metabolites of the strains showed good antiproliferative effect on Caco-2, while lower amounts showed good antiproliferative effect on the HT-29 cell line. When all the data were considered, it was determined that the strains were close to each other, but the YAAS 23 strain showed slightly better properties. In conclusion, breast milk is a unique environment harboring beneficial bacteria such as L. plantarum for human health.

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从母乳中新分离出的植物乳杆菌菌株的益生特性和功能以及代谢产物的增殖抑制潜力。

研究人员评估了从土耳其人的母乳中新分离和鉴定出的四株植物乳杆菌（Lactiplantibacillus plantarum）对结肠癌细胞株的益生、功能和代谢物增殖抑制潜力。在模拟胃和肠道培养基中，所有菌株都表现出很强的益生特性，表现出抗药性，但随着时间和浓度的增加而降低。这些菌株对青霉素 G、利福平和氯霉素敏感，对所有致病菌都有抗菌作用。菌株中未检测到柠檬酸、苹果酸、酒石酸、丙酮酸和富马酸，而乙酸的含量最高。外多糖（EPS）的定量-定性分析和结构特征得到证实，并确定菌株合成的外多糖数量相似。与标准抗氧化剂相比，菌株的 DPPH 活性较低，ABTS 活性相似。高含量的菌株代谢物对 Caco-2 细胞系具有良好的抗增殖作用，而低含量的菌株代谢物对 HT-29 细胞系具有良好的抗增殖作用。综合所有数据，可以确定这些菌株的性能接近，但 YAAS 23 菌株的性能略胜一筹。总之，母乳是一个独特的环境，其中蕴藏着植物乳杆菌等对人体健康有益的细菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.