Exploration of the Active Components and Mechanism of Jiegeng (Platycodonis Radix) in the Treatment of Influenza Virus Pneumonia Through Network Pharmacology Analysis and Experimental Verification

IF 3.2 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhiying Feng, Kangyu Wang, Jiawang Huang, Zhuolin Liu, Jingmin Fu, Jianing Shi, Xinyue Ma, Ling Li, Qiong Wu
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引用次数: 0

Abstract

This study aimed to explore the pathogenesis of platycodin D and luteolin, which are both active components in Jiegeng (Platycodonis Radix), in the treatment of influenza virus pneumonia through network pharmacology analysis combined with experimental verification. The bioactive components of Jiegeng (Platycodonis Radix) were screened by TCMSP and literature mining, and the results were standardized via the UniProt database. The action targets for the disease were identified from databases including OMIM, GeneCards, TTD, DisGeNET, and PharmGKB. Then, the visualized key target regulatory network and protein–protein interaction (PPI) network for the active components were established using Cytoscape3.7.1 software. The findings were illustrated through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The intervention concentrations of platycodin D and luteolin were screened by the CCK8 method, and the important signaling pathways of platycodin D and luteolin for treating influenza virus pneumonia were verified by RT-qPCR and Western blot tests. From data mining, 89 common drug-disease targets were screened out, and five major active components of Jiegeng (Platycodonis Radix), including platycodin D and luteolin, were obtained. Besides, 11 therapeutic targets including IL-17, IL-6, TNF-α, JUN, and MAKP1 were identified by PPI network analysis. GO and KEGG enrichment analyses showed that the pathways most related to the mechanisms of Jiegeng (Platycodonis Radix) against influenza virus pneumonia included the TNF and IL-17 signaling pathways and apoptosis. In vitro experiments demonstrated that the model group exhibited a notable elevation in mRNA levels of IL-6, IL-17, TNF-α, JUN, MAPK1, and the IL-17/−acting protein ratio, as compared to the control group (p < 0.05). In contrast to the model group, the IL-6, IL-17, TNF-α, JUN, MAPK1 mRNA expression levels, and the IL-17 protein ratio in both the platycodin D group and luteolin group were considerably decreased (p < 0.05). Combined with network pharmacology and experimental verification, this study revealed that platycodin D and luteolin in Jiegeng (Platycodonis Radix) may treat influenza virus pneumonia by regulating inflammation through the IL-17 signaling pathway.

通过网络药理学分析和实验验证探索鸡血藤治疗流感病毒肺炎的活性成分和机理
本研究旨在通过网络药理学分析结合实验验证,探讨桔梗中的有效成分桔梗皂苷D和叶黄素在治疗流感病毒肺炎中的作用机理。通过TCMSP和文献挖掘筛选桔梗的生物活性成分,并通过UniProt数据库对结果进行标准化。从 OMIM、GeneCards、TTD、DisGeNET 和 PharmGKB 等数据库中确定了疾病的作用靶点。然后,使用 Cytoscape3.7.1 软件建立了可视化的关键靶点调控网络和活性成分的蛋白质-蛋白质相互作用(PPI)网络。研究结果通过基因本体(GO)和京都基因组百科全书(KEGG)富集分析进行了说明。通过CCK8法筛选了桔梗皂苷D和木犀草素的干预浓度,并通过RT-qPCR和Western印迹检测验证了桔梗皂苷D和木犀草素治疗流感病毒肺炎的重要信号通路。通过数据挖掘,筛选出89个常见的药物-疾病靶点,获得了桔梗中的5种主要活性成分,包括桔梗皂苷D和木犀草素。此外,通过PPI网络分析还发现了IL-17、IL-6、TNF-α、JUN和MAKP1等11个治疗靶点。GO和KEGG富集分析表明,与桔梗抗流感病毒肺炎机制最相关的通路包括TNF和IL-17信号通路以及细胞凋亡。体外实验表明,与对照组相比,模型组的 IL-6、IL-17、TNF-α、JUN、MAPK1 的 mRNA 水平和 IL-17/ 作用蛋白比值均有明显升高(p<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chemical Biology & Drug Design
Chemical Biology & Drug Design 医学-生化与分子生物学
CiteScore
5.10
自引率
3.30%
发文量
164
审稿时长
4.4 months
期刊介绍: Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.
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