The goat PRLR gene: mRNA expression, association analysis of InDel variants with body weight and growth traits

IF 2.6 3区 生物学 Q2 GENETICS & HEREDITY
Gene Pub Date : 2024-11-08 DOI:10.1016/j.gene.2024.149081
Congliang Wang , Zhaofei Ren , Xiaoyu Liu , Xiaoyue Song , Lei Shi , Huaiyan Kang , Dong Ma , Xianyong Lan , Lei Qu , Haijing Zhu , Jinlian Hua
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Abstract

Prolactin is a single-chain peptide hormone produced by the anterior pituitary gland and plays an important role in the reproductive development of mammals. Therefore, we investigated the expression of the PRLR gene in various goat tissues during distinct developmental stages, as well as the correlation between the 16 bp InDel and growth traits in goats. The study findings demonstrated a consistent expression and distribution of PRLR in both yearling and adult goat tissues, with the highest levels observed in the testis. Association analysis showed that in the yearling IMWC goat population (n = 463), this 16 bp InDel variation was associated with body weight (P = 0.026), body height (P = 0.005), body length (P = 0.008), heart girth (P = 0.001), chest depth (P = 0.000075), and chest width (P = 0.005); In the adult IMWC goat population (n = 211), the 16 bp InDel variation was associated with body height (P = 0.011), height across the hip (P = 0.0003), chest depth (P = 0.001), and cashmere fineness (P = 0.022); In all IMWC goat populations (n = 674), this 16 bp InDel was related to body weight (P = 0.042), body height (P = 0.001), body length (P = 0.013), heart girth (P = 0.012), height across the hip (P = 0.038), chest depth (P = 0.001), and chest width (P = 0.006). The bioinformatics analysis has revealed that the significant impact of a 16 bp InDel on growth traits in goats may be attributed to its specific binding to the transcription factors AP-2α and Sp1. These findings suggest that this genetic variant plays a crucial role in the growth and development of goats, making it a valuable DNA marker for selecting goats with superior growth traits.
山羊 PRLR 基因:mRNA 表达、InDel 变异与体重和生长性状的关联分析。
催乳素是一种由垂体前叶分泌的单链肽类激素,在哺乳动物的生殖发育过程中发挥着重要作用。因此,我们研究了不同发育阶段山羊不同组织中 PRLR 基因的表达,以及 16 bp InDel 与山羊生长性状的相关性。研究结果表明,PRLR 在一岁山羊和成年山羊组织中的表达和分布一致,在睾丸中的表达水平最高。关联分析表明,在一岁的 IMWC 山羊群体(n = 463)中,16 bp InDel 变异与体重(P = 0.026)、体高(P = 0.005)、体长(P = 0.008)、心宽(P = 0.001)、胸深(P = 0.000075)和胸宽(P = 0.005);在成年 IMWC 山羊群体(n = 211)中,16 bp InDel 变异与体高(P = 0.011)、跨臀高(P = 0.0003)、胸深(P = 0.001)和羊绒细度(P = 0.022)相关;在所有 IMWC 山羊群体(n = 674)中,16 bp InDel 与体重(P = 0.042)、体高(P = 0.001)、体长(P = 0.013)、心宽(P = 0.012)、跨髋高(P = 0.038)、胸深(P = 0.001)和胸宽(P = 0.006)有关。生物信息学分析表明,16 bp InDel 对山羊生长性状的显著影响可能是由于它与转录因子 AP-2α 和 Sp1 的特异性结合。这些研究结果表明,该基因变异在山羊的生长发育过程中起着至关重要的作用,使其成为筛选具有优良生长性状的山羊的重要 DNA 标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Gene
Gene 生物-遗传学
CiteScore
6.10
自引率
2.90%
发文量
718
审稿时长
42 days
期刊介绍: Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.
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