Regulation of cell-mediated immune responses in dairy bulls via long non-coding RNAs from submandibular lymph nodes, peripheral blood, and the spleen

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2024-11-01 DOI:10.1016/j.ygeno.2024.110958

Xiuxin Zhao , Xiao Wang , Guanghui Xue , Yundong Gao , Yuanpei Zhang , Yanqin Li , Yachun Wang , Jianbin Li

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引用次数: 0

Abstract

Cell-mediated immune responses (CMIRs) are critical to building a robust immune system and reducing disease susceptibility in cattle. Long non-coding RNAs (lncRNAs) regulate various biological processes. However, to the best of our knowledge, the characterization and functions of lncRNAs and their regulations on the bovine CMIR have not been investigated comprehensively. In this study, experimental bulls were immunized with heat-killed preparation of Candida albicans (HKCA) to induce delayed-type hypersensitivity (DTH). Three bulls were classified as high- CMIR responders and three were low-CMIR responders, based on their classical DTH skin reactions. LncRNAs were identified in the submandibular lymph nodes, peripheral blood, and spleen of high- and low-CMIR animals using strand-specific RNA sequencing. A total of 21,003 putative lncRNAs were identified across tissues, and 420, 468, and 599 lncRNAs were differentially expressed between the two groups in the submandibular lymph node, peripheral blood, and spleen tissues, respectively. Functional analysis of the differentially expressed lncRNA (DElncRNA) target genes showed that a number of immune-related Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were enriched, including immune response, cell adhesion, nucleosome, DNA packaging, antigen processing and presentation, and complement and coagulation cascades. Tissue specificity analysis indicated that lncRNA transcripts have stronger tissue specificity than mRNA. Furthermore, an interaction network was constructed based on DElncRNAs and DEGs, and 11, 14, and 11 promising lncRNAs were identified as potential candidate genes influencing immune response regulation in submandibular lymph nodes, peripheral blood, and spleen tissues, respectively. These results provide a foundation for further research into the biological functions of lncRNAs associated with bovine CMIR and identify candidate lncRNA markers for cell-mediated immune responses.

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通过颌下淋巴结、外周血和脾脏中的长非编码 RNA 调节奶牛的细胞介导免疫反应。

细胞介导的免疫应答（CMIRs）对于建立强大的免疫系统和降低牛的疾病易感性至关重要。长非编码 RNA（lncRNA）可调控各种生物过程。然而，据我们所知，lncRNA 的特征和功能及其对牛 CMIR 的调控尚未得到全面研究。本研究用白念珠菌热处理制剂（HKCA）对实验公牛进行免疫，以诱导迟发型超敏反应（DTH）。根据其典型的 DTH 皮肤反应，三头公牛被归类为高 CMIR 反应者，三头为低 CMIR 反应者。利用链特异性 RNA 测序鉴定了高 CMIR 和低 CMIR 动物颌下淋巴结、外周血和脾脏中的 LncRNA。在各组织中共鉴定出 21,003 个推测的 lncRNA，在两组动物的颌下淋巴结、外周血和脾脏组织中，分别有 420、468 和 599 个 lncRNA 存在差异表达。对差异表达的lncRNA（DElncRNA）靶基因进行的功能分析显示，富集了许多与免疫相关的基因本体（GO）术语和京都基因组百科全书（KEGG）通路，包括免疫反应、细胞粘附、核小体、DNA包装、抗原加工和递呈以及补体和凝血级联。组织特异性分析表明，与 mRNA 相比，lncRNA 转录本具有更强的组织特异性。此外，基于DElncRNA和DEGs构建了一个相互作用网络，并分别鉴定出11、14和11个有希望的lncRNA作为影响颌下淋巴结、外周血和脾脏组织免疫应答调控的潜在候选基因。这些结果为进一步研究与牛CMIR相关的lncRNA的生物学功能以及鉴定细胞介导免疫反应的候选lncRNA标记物奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.