Intergenerational effects of maternal rate of body weight gain on the multi-omics hepatic profiles of bovine fetuses

IF 2.6 3区 生物学 Q2 GENETICS & HEREDITY
Gene Pub Date : 2024-11-12 DOI:10.1016/j.gene.2024.149082
Muhammad Anas , Alison K. Ward , Kacie L. McCarthy , Pawel P. Borowicz , Lawrence P. Reynolds , Joel S. Caton , Carl R. Dahlen , Wellison J.S. Diniz
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引用次数: 0

Abstract

Maternal periconceptual nutrition affects the growth trajectory of developing fetuses by modulating gene expression. The regulatory mechanisms and their role in fetal development remain underexplored in livestock models. Herein, we investigated the effects of maternal rate of body weight (BW) gain during early gestation on the DNA methylation, microRNA profiles, and their interaction with the hepatic gene expression in female fetuses. At breeding, 36 crossbred beef heifers (∼13 months of age) were randomly assigned to a nutritional plane to gain Low (0.28 kg/day; LG, n = 18) or Moderate (0.79 kg/day; MG, n = 18) BW through the first 83 days of gestation. A subset of pregnant heifers (n = 17) was selected, and fetal liver samples were collected on day 83 of gestation for DNA methylation and miRNA-Sequencing. After data quality control, miRDeep2 and Bismark tools were used to analyze miRNA and methylation data, respectively. The bta-miR-206 was the only differentially expressed miRNA (FDR = 0.02). Eight differentially methylated genes were identified (DMGs, FDR < 0.1). The over-represented pathways and biological processes (adj. p < 0.05) for bta-miR-206 targeted genes were associated with embryonic development, energy metabolism, and mineral transport, whereas the DMGs regulated anatomical structural development and transcriptional regulation. Our results show that key genes involved with liver metabolism, tissue structure, and function were regulated by DNA methylation and the miR-206. However, further investigation is warranted to determine physiological responses and long-term consequences on animal performance.
母体体重增加率对牛胎儿肝脏多组学特征的代际影响
母体围产期营养通过调节基因表达影响发育中胎儿的生长轨迹。家畜模型中的调控机制及其在胎儿发育中的作用仍未得到充分探索。在此,我们研究了妊娠早期母体体重(BW)增长速度对雌性胎儿 DNA 甲基化、microRNA 图谱的影响及其与肝脏基因表达的相互作用。36头杂交肉用小母牛(13个月大)在配种时被随机分配到一个营养平面中,在妊娠期的前83天中增加低体重(0.28千克/天;LG,n = 18)或中等体重(0.79千克/天;MG,n = 18)。选取一部分怀孕母牛(n = 17),在妊娠第 83 天采集胎儿肝脏样本,进行 DNA 甲基化和 miRNA 序列测定。数据质量控制后,miRDeep2 和 Bismark 工具分别用于分析 miRNA 和甲基化数据。bta-miR-206 是唯一有差异表达的 miRNA(FDR = 0.02)。发现了 8 个差异甲基化基因(DMG,FDR < 0.1)。代表性过高的通路和生物过程(adj.
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来源期刊
Gene
Gene 生物-遗传学
CiteScore
6.10
自引率
2.90%
发文量
718
审稿时长
42 days
期刊介绍: Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.
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