Hsa_circ_0000423 promotes colorectal cancer EMT and immune escape by competitive adsorption of miR-369-3p mediating CCND1 expression.

IF 2.8 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Discover. Oncology Pub Date : 2024-11-09 DOI:10.1007/s12672-024-01501-3

TianFu Huang, KaiHai Jiang, LinTao Li, GuangSheng Li, YuSheng Cao, XuSen Huang

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引用次数: 0

Abstract

Background: This investigation evaluated the mechanism of hsa_circ_0000423 in colorectal cancer (CRC).

Methods: The hsa_circ_0000423 gene was identified by bioinformatics analyses of GEO circRNA microarrays, and its expression in CRC was investigated. Based on this, in vitro experiments were conducted. Assays with dual luciferase reporter and RIP were conducted to detect interactions between hsa_circ_0000423, miR-369-3p and CCND1. Cell proliferation was measured by MTT and colony formation assay assays, apoptosis was detected by flow cytometry, migration and invasion were detected by Transwell, and expression of EMT-related proteins was detected by Western Blot. SW480 cells and T cells were co-cultured to assess immune escape.

Results: hsa_circ_0000423 and CCND1 were elevated in CRC while miR-369-3p was downregulated Silencing hsa_circ_0000423 resulted in reduced CCND1 expression by upregulating miR-369-3p. Overexpressing CCND1 or down-regulating miR-369-3p both interrupted the anti-tumor role of silencing hsa_circ_0000423 on CRC cells.

Conclusion: Hsa_circ_0000423 promotes CCND1 expression through competitive binding of miR-369-3p and promotes CRC cell development and immune escape.

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Hsa_circ_0000423通过竞争性吸附介导 CCND1 表达的 miR-369-3p 来促进结直肠癌 EMT 和免疫逃逸。

背景：本研究评估了 hsa_circ_0000423 在结直肠癌中的作用机制：本研究评估了 hsa_circ_0000423 在结直肠癌（CRC）中的作用机制：方法：通过对 GEO circRNA 微阵列进行生物信息学分析，确定了 hsa_circ_0000423 基因，并研究了该基因在 CRC 中的表达。在此基础上进行了体外实验。使用双荧光素酶报告器和 RIP 进行检测，以发现 hsa_circ_0000423、miR-369-3p 和 CCND1 之间的相互作用。细胞增殖通过 MTT 和集落形成试验测定，细胞凋亡通过流式细胞术检测，迁移和侵袭通过 Transwell 检测，EMT 相关蛋白的表达通过 Western 印迹检测。结果：hsa_circ_0000423 和 CCND1 在 CRC 中升高，而 miR-369-3p 下调沉默 hsa_circ_0000423 会通过上调 miR-369-3p 导致 CCND1 表达减少。过表达 CCND1 或下调 miR-369-3p 都会中断沉默 hsa_circ_0000423 对 CRC 细胞的抗肿瘤作用：结论：Hsa_circ_0000423通过竞争性结合miR-369-3p促进CCND1的表达，并促进CRC细胞的发育和免疫逃逸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Discover. Oncology Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

2.40

自引率

9.10%

发文量

122

审稿时长

5 weeks