Cationic lipid transfection induces nuclear actin filaments

IF 2.5 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

Cytometry Part A Pub Date : 2024-11-11 DOI:10.1002/cyto.a.24903

Molika Sinha, Maria Kristha Fernandez, Malte Renz

引用次数: 0

Abstract

Cationic lipids are widely used for gene delivery. Here, we report the transient formation of nuclear actin filaments in mammalian cells transfected with commercially available transfection reagents regardless of the proteins transfected. Readily detectable with phalloidin, nuclear actin ranges from short filaments to a fully developed network. Nuclear actin filaments persist for hours, peak 20 h after transfection, and may be involved in DNA damage repair.

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阳离子脂质转染可诱导核肌动蛋白丝。

阳离子脂质被广泛用于基因递送。在这里，我们报告了用市售转染试剂转染的哺乳动物细胞中核肌动蛋白丝的瞬时形成，与转染的蛋白质无关。核肌动蛋白可随时用类黄嘌呤检测到，从短丝到完全发育的网络都有。核肌动蛋白丝持续存在数小时，在转染后 20 小时达到峰值，可能参与 DNA 损伤修复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytometry Part A 生物-生化研究方法

CiteScore

8.10

自引率

13.50%

发文量

183

审稿时长

4-8 weeks

期刊介绍： Cytometry Part A, the journal of quantitative single-cell analysis, features original research reports and reviews of innovative scientific studies employing quantitative single-cell measurement, separation, manipulation, and modeling techniques, as well as original articles on mechanisms of molecular and cellular functions obtained by cytometry techniques. The journal welcomes submissions from multiple research fields that fully embrace the study of the cytome: Biomedical Instrumentation Engineering Biophotonics Bioinformatics Cell Biology Computational Biology Data Science Immunology Parasitology Microbiology Neuroscience Cancer Stem Cells Tissue Regeneration.