Mathematical Modeling and Simulation of 1,3‐Propanediol Production by Klebsiella pneumoniae BLh‐1 in a Batch Bioreactor Using Bayesian Statistics

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nathalia Lobato Moraes, Mailson Batista de Vilhena, Daniele Misturini Rossi, Bruno Marques Viegas
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Abstract

Mathematical modeling and computer simulation are fundamental for optimizing biotechnological processes, enabling cost reduction and scalability, thereby driving advancements in the bioindustry. In this work, mathematical modeling and estimation of fermentative kinetic parameters were carried out to produce 1,3‐propanediol (1,3‐PDO) from residual glycerol and Klebsiella pneumoniae BLh‐1. The Markov chain Monte Carlo method, using the Metropolis‐Hastings algorithm, was applied to experimental data from a batch bioreactor under aerobic and anaerobic conditions. Sensitivity analysis and parameter evolution studies were conducted. The root‐mean‐square error (rRMSE) was chosen as the validation and calibration metric for the developed mathematical model. The results indicated that the average tolerance of glycerol was 174.68 and 44.85 g L−1, the inhibitory products was 150.95 g L−1 for ethanol and 35.56 g L−1 for 1,3‐PDO, and the maximum specific rate of cell growth was 0.189 and 0.275 h−1, for aerobic and anaerobic cultures, respectively. The model presented excellent fits in both crops, with rRMSE values between 0.09 − 33.74% and 3.58 − 31.82%, for the aerobic and anaerobic environment, respectively. With this, it was possible to evaluate and extract relevant information for a better understanding and control of the bioprocess.

Abstract Image

利用贝叶斯统计对批式生物反应器中肺炎克雷伯氏菌 BLh-1 生产 1,3-丙二醇的过程进行数学建模和模拟
数学建模和计算机模拟是优化生物技术过程、降低成本和提高可扩展性的基础,从而推动生物产业的进步。在这项工作中,对利用残余甘油和肺炎克雷伯氏菌 BLh-1 生产 1,3-丙二醇(1,3-PDO)的发酵动力学参数进行了数学建模和估算。使用 Metropolis-Hastings 算法的马尔科夫链蒙特卡罗方法被应用于有氧和厌氧条件下批量生物反应器的实验数据。进行了敏感性分析和参数演变研究。选择均方根误差(rRMSE)作为所开发数学模型的验证和校准指标。结果表明,在有氧和厌氧培养条件下,甘油的平均耐受量分别为 174.68 和 44.85 g L-1,乙醇的抑制产物为 150.95 g L-1,1,3-PDO 的抑制产物为 35.56 g L-1,细胞生长的最大比速率分别为 0.189 和 0.275 h-1。该模型对两种作物的拟合效果都很好,有氧环境和厌氧环境的 rRMSE 值分别为 0.09 - 33.74% 和 3.58 - 31.82%。因此,可以对相关信息进行评估和提取,以便更好地理解和控制生物过程。
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来源期刊
Biotechnology and Bioengineering
Biotechnology and Bioengineering 工程技术-生物工程与应用微生物
CiteScore
7.90
自引率
5.30%
发文量
280
审稿时长
2.1 months
期刊介绍: Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.
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