Genotoxicity analysis of a flame retardant, aluminum diethylphosphinate

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-11-01 DOI:10.1016/j.mrgentox.2024.503829

T.O.L. Leoncio , A.S. Fernandes , I. Felzenszwalb , C.F. Araujo-Lima , D.P. Oliveira , D.J. Dorta , C.F. Sampaio , E.R.A. Ferraz

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Abstract

Flame retardants, crucial for fire prevention, are used worldwide, but they are considered to be ‘emerging contaminants’ and may pose risks to human and environmental health. Aluminum diethyl phosphinate (ALPI) is a halogen-free flame retardant. To evaluate the toxicity of this compound, the following assays were performed: Salmonella/microsome mutagenicity assay; toxicity assays with two endpoints (mitochondrial dehydrogenase activity, plasma membrane integrity); micronucleus assay with human hepatoma cell line HepG2. ALPI was not mutagenic in Salmonella strains TA97, TA98, TA100, TA102, or TA104. ALPI was not cytotoxic at any concentration tested. The HepG2 micronucleus assay showed genotoxicity of ALPI at 200 µg/mL and no cytotoxicity (cytokinesis-block proliferation index, CBPI). Our data are relevant to the regulation of flame retardants.

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阻燃剂二乙基膦酸铝的遗传毒性分析

阻燃剂对防火至关重要，在全球范围内得到广泛应用，但它们被认为是 "新出现的污染物"，可能对人类和环境健康造成危害。二乙基膦酸铝（ALPI）是一种无卤阻燃剂。为了评估这种化合物的毒性，我们进行了以下检测：沙门氏菌/微粒体致突变试验；两个终点（线粒体脱氢酶活性、质膜完整性）的毒性试验；人肝癌细胞系 HepG2 的微核试验。ALPI 对沙门氏菌菌株 TA97、TA98、TA100、TA102 或 TA104 均无诱变作用。在任何测试浓度下，ALPI 都没有细胞毒性。HepG2 微核试验显示，ALPI 在 200 微克/毫升时具有遗传毒性，而在 200 微克/毫升时没有细胞毒性（细胞分裂阻滞增殖指数，CBPI）。我们的数据与阻燃剂的监管有关。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.