Calculated globulin can be used as a screening test for antibody deficiency in children and adolescents.

IF 5.7 2区医学 Q1 IMMUNOLOGY

Frontiers in Immunology Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI:10.3389/fimmu.2024.1495564

Cristina Frias Sartorelli de Toledo Piza, Carolina Sanchez Aranda, Dirceu Solé, Stephen Jolles, Antonio Condino-Neto

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引用次数: 0

Abstract

Purpose: Calculated globulin (CG, total protein minus albumin levels) correlate well with IgG levels and has been proposed as a suitable screening method for individuals with primary antibody deficiencies (PADs). We aimed to show the correlation of CG with IgG levels in children and adolescents, utilizing a common method for albumin measurement, bromocresol green.

Methods: Individuals from two Allergy and Immunology clinics were invited to participate. Inclusion criteria were age < 18, stable conditions, and signed informed consent. We included 1084 individuals. Immunoglobulin G values were determined by immunoturbidimetry; the colorimetric bromocresol green method and the Architect Biuret method were utilized for the albumin and total protein (TP) measurements, respectively.

Results: A total of 1084 individuals were included in the analysis and divided into 4 age groups (0 to <1 year, 1 to <4 years, 4 to <10 years, and 10 to <18 years). For all patients, the mean age was 6.1 (± 5) years old, the mean IgG was 9.4 (± 4.7) g/L, and CG was 23.7 (± 5.9) g/L. The most frequent diagnosis were respiratory allergies, followed by inborn errors of immunity. IgG and CG varied according to age group. Cutoff values for hypogammaglobulinemia varied from 13.8 g/L in children < 1 year to 23.1 g/L in children and adolescents aged 10 to <18 years. CG sensitivity varied from 70.9% in children aged 1 to <4 years old to 95.8% in children 4 to <10. Specificity ranged from 87.5% in children 4 to <10 years old to 100% in children and adolescents aged 10 to <18 years.

Conclusion: CG is a suitable screening test for hypogammaglobulinemia in children less than 18 years of age.

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计算球蛋白可用作儿童和青少年抗体缺乏症的筛查试验。

目的：计算球蛋白（CG，总蛋白减去白蛋白的水平）与 IgG 水平有很好的相关性，被认为是筛查原发性抗体缺乏症（PAD）患者的合适方法。我们的目的是利用白蛋白测量的常用方法溴甲酚绿来显示儿童和青少年中 CG 与 IgG 水平的相关性：方法：邀请两家过敏与免疫学诊所的患者参加。纳入标准为年龄小于 18 岁、病情稳定并签署知情同意书。我们共纳入了 1084 人。免疫球蛋白 G 值采用免疫比浊法测定；白蛋白和总蛋白 (TP) 的测定分别采用比色溴甲酚绿法和 Architect Biuret 法：共有 1084 人参与了分析，并被分为 4 个年龄组（0 岁至 15 岁）：CG 是筛查 18 岁以下儿童低丙种球蛋白血症的合适检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Immunology IMMUNOLOGY-

CiteScore

9.80

自引率

11.00%

发文量

7153

审稿时长

14 weeks

期刊介绍： Frontiers in Immunology is a leading journal in its field, publishing rigorously peer-reviewed research across basic, translational and clinical immunology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Immunology is the official Journal of the International Union of Immunological Societies (IUIS). Encompassing the entire field of Immunology, this journal welcomes papers that investigate basic mechanisms of immune system development and function, with a particular emphasis given to the description of the clinical and immunological phenotype of human immune disorders, and on the definition of their molecular basis.