ITGA1 Promotes Glioma Cell Proliferation and Affects Immune Cell Infiltration in Low-Grade Glioma.

IF 4.4 3区 医学 Q2 CELL BIOLOGY
Mediators of Inflammation Pub Date : 2024-10-29 eCollection Date: 2024-01-01 DOI:10.1155/2024/6147483
Yanhong Ren, Jianchang Xu, Zhengkui Zhang, Rutong Yu
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Abstract

Background: Low-grade glioma (LGG) is a commonly occurring type of central nervous system cancer. Integrin α1 (ITGA1), a family member of integrins, is implied in the malignant development of cancers, but the fundamental role of ITGA1 has not been illustrated yet in glioma. This study aimed to evaluate the prognostic value of ITGA1. Methods: Correlations between ITGA1 and relevant clinical features were analyzed in the LGG datasets based on Chinese Glioma Genome Atlas (CGGA) and Tumor Genome Atlas (TCGA). Glioma clinical samples and glioma cell lines were identified at the level of mRNA and protein level by Western blot. Cox regression were developed to assess the involvement of ITGA1 expression in predicting survival in LGG patients. Application of GSEA enrichment analysis to reveal ITGA1-mediated biological functions in LGG. Using TIMER 2.0 to analyze correlations between immune cell infiltration. In addition, ITGA1 high expression was analyzed for correlation with immune checkpoint-related genes and cumulative survival time. Results: ITGA1 was significantly more expressed in LGG than in normal samples. Cox regression indicated that ITGA1 was a risk factor independently for prognosis in LGG patients. GSEA enrichment analysis indicated that ITGA1 was engaged in several immunomodulatory processes. ITGA1 expression was shown to be highly correlated with the immune score, stromal score, and estimate score in LGG. ITGA1 was significantly related to the immune checkpoint-associated gene expression. In vivo experiments showed that overexpression of ITGA1 promoted glioma cell invasion. Conclusion: High ITGA1 expression is correlated with immune infiltration of the low-grade tumor, leading to poor prognoses in LGG patients.

ITGA1 促进胶质瘤细胞增殖并影响低级别胶质瘤的免疫细胞浸润
背景:低级别胶质瘤(LGG)是一种常见的中枢神经系统癌症。整合素α1(ITGA1)是整合素家族的成员之一,在癌症的恶性发展过程中起着重要作用,但在胶质瘤中,ITGA1的基本作用尚未得到证实。本研究旨在评估 ITGA1 的预后价值。方法:基于中国胶质瘤基因组图谱(CGGA)和肿瘤基因组图谱(TCGA)的LGG数据集分析了ITGA1与相关临床特征之间的相关性。通过Western blot对胶质瘤临床样本和胶质瘤细胞系的mRNA和蛋白质水平进行鉴定。通过Cox回归评估ITGA1表达在预测LGG患者生存率中的作用。应用GSEA富集分析揭示ITGA1在LGG中介导的生物学功能。使用 TIMER 2.0 分析免疫细胞浸润之间的相关性。此外,还分析了 ITGA1 高表达与免疫检查点相关基因和累积生存时间的相关性。结果显示ITGA1在LGG中的表达明显高于正常样本。Cox回归表明,ITGA1是影响LGG患者预后的独立危险因素。GSEA富集分析表明,ITGA1参与了多个免疫调节过程。ITGA1的表达与LGG的免疫评分、基质评分和估计评分高度相关。ITGA1 与免疫检查点相关基因的表达密切相关。体内实验表明,ITGA1的过表达会促进胶质瘤细胞的侵袭。结论ITGA1的高表达与低级别肿瘤的免疫浸润相关,导致LGG患者预后不良。
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来源期刊
Mediators of Inflammation
Mediators of Inflammation 医学-免疫学
CiteScore
8.70
自引率
0.00%
发文量
202
审稿时长
4 months
期刊介绍: Mediators of Inflammation is a peer-reviewed, Open Access journal that publishes original research and review articles on all types of inflammatory mediators, including cytokines, histamine, bradykinin, prostaglandins, leukotrienes, PAF, biological response modifiers and the family of cell adhesion-promoting molecules.
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