Transcriptome analysis of mammary epithelial cell between Sewa sheep and East FriEsian sheep from different localities.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Rui Li, Junru Pan, Cheng Pan, Jingjing Li, Zhenzhen Zhang, Khuram Shahzad, Yu Sun, Quzhu Yixi, Wangjie Zhaxi, Haofeng Qing, Tianzeng Song, Wangsheng Zhao
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Abstract

Mammary epithelial cells, the only milk-producing cell type in the mammary gland, undergo dynamic proliferation and differentiation during pregnancy, culminating in lactation postpartum. The East FriEsian sheep ranks among the world's most prolific dairy breeds, while the Sewa sheep, a unique dual-purpose breed autochthonous to the Qinghai-Tibet Plateau, exhibits significantly lower milk production. Employing tissue culture methods, we successfully established mammary epithelial cell lines from both breeds. Morphological assessment of mammary epithelial cells and immunofluorescence identification of Cytokeratin 7 and Cytokeratin 8 confirmed the epithelial identity of the isolated cells. Subsequent RNA-seq analysis of these in vitro epithelial cell lines revealed 1813 differentially expressed genes (DEGs). Among these, 1108 were significantly up-regulated and 705 were down-regulated in Sewa epithelial sheep cells compared to East FriEsian epithelial cells. KEGG enrichment analysis identified cellular processes, environmental information processing, human diseases, metabolism, and organismal systems as the primary functional categories associated with DEGs. Gene ontology (GO) terms annotation, categorized into molecular function, biological processes, and cellular component, yielded "binding and catalytic activity," "molecular function regulator activity," and "cellular process," "biological regulation," and "regulation of biological process" as the top three terms within each domain, respectively. Clusters of Orthologous Groups of proteins (KOG) classification further revealed that "signal transduction mechanisms" accounted for the largest proportion of DEGs among all KOG categories. Finally, based on these analyses, ATF3 and MPP7 were identified as promising candidate genes for regulating lactation.

来自不同地区的苏瓦绵羊和东弗里西亚绵羊乳腺上皮细胞的转录组分析。
乳腺上皮细胞是乳腺中唯一的产奶细胞类型,在怀孕期间经历动态增殖和分化,最终在产后泌乳。东弗里西亚绵羊是世界上最多产的奶羊品种之一,而青藏高原特有的两用绵羊品种苏瓦绵羊的产奶量却明显较低。我们采用组织培养方法,成功建立了这两个品种的乳腺上皮细胞系。乳腺上皮细胞的形态学评估以及细胞角蛋白 7 和细胞角蛋白 8 的免疫荧光鉴定证实了分离细胞的上皮特性。随后对这些体外上皮细胞系进行的 RNA-seq 分析发现了 1813 个差异表达基因(DEGs)。与East FriEsian上皮细胞相比,其中1108个基因明显上调,705个基因明显下调。KEGG富集分析发现,细胞过程、环境信息处理、人类疾病、新陈代谢和机体系统是与DEGs相关的主要功能类别。基因本体论(GO)术语注释分为分子功能、生物过程和细胞成分三类,每个领域的前三个术语分别是 "结合和催化活性"、"分子功能调节活性 "以及 "细胞过程"、"生物调控 "和 "生物过程调控"。蛋白质同源群(KOG)分类进一步显示,在所有 KOG 类别中,"信号转导机制 "占 DEGs 的比例最大。最后,根据上述分析,ATF3 和 MPP7 被确定为有望调控泌乳的候选基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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