Immune response to topical sodium lauryl sulfate differs from classical irritant and allergic contact dermatitis

IF 4.5 3区 医学 Q2 IMMUNOLOGY
Marvin Nüsken, Fabian Heinemeier, Silke Sabina Matzke, Patryk Porebski, Susann Forkel, Prasad Dasari, Andrea Braun, Andreas Erich Zautner, Michael Peter Schön, Timo Buhl
{"title":"Immune response to topical sodium lauryl sulfate differs from classical irritant and allergic contact dermatitis","authors":"Marvin Nüsken,&nbsp;Fabian Heinemeier,&nbsp;Silke Sabina Matzke,&nbsp;Patryk Porebski,&nbsp;Susann Forkel,&nbsp;Prasad Dasari,&nbsp;Andrea Braun,&nbsp;Andreas Erich Zautner,&nbsp;Michael Peter Schön,&nbsp;Timo Buhl","doi":"10.1002/eji.202350798","DOIUrl":null,"url":null,"abstract":"<p>Sodium lauryl sulfate (SLS) is used as a control irritant in patch testing for allergic contact dermatitis (ACD). However, up to 20% of those tested react to SLS, whereby the pathophysiological basis of this reaction is still unclear. To mimic patch test reactions, we repeatedly applied SLS to the skin of wild-type mice. Reactions were compared with those in a classical ACD model induced by oxazolone and an irritant contact dermatitis (ICD) model induced by croton oil. Skin inflammation was assessed with ear thickness measurements, immunohistochemistry, qRT-PCR, and flow cytometry. Topical SLS treatment was further investigated in <i>Flg/Hrnr</i><sup>−/−</sup>, <i>Myd88/Tlr3</i><sup>−/−</sup>, and <i>Rag1</i><sup>−/−</sup> mouse models. All three compounds caused ear swelling with different courses. Oxazolone treatment, compared with the ICD model, resulted in a greater influx of immune cells (CD4<sup>+</sup>, MHCII<sup>+</sup>, CD11b<sup>+</sup>). Similarly, SLS did not induce immune cell infiltration or expression of selected inflammatory and regulatory cytokines. SLS induced the most pronounced keratinocyte proliferation. Compared with wild-type mice, topical SLS application did not increase ear swelling in skin barrier deficient <i>Flg/Hrnr</i><sup>−/−</sup> mice, but led to significantly delayed swelling in mice with defects in innate or adaptive immune functions (<i>Myd88/Tlr3</i><sup>−/−</sup>, <i>Rag1</i><sup>−/−</sup>). SLS-induced contact dermatitis differed from classical ACD and ICD, as it elicited less pronounced immune alterations. Skin barrier impairment does not affect SLS-induced contact dermatitis, whereas both innate and adaptive components are involved in SLS skin reactions.</p>","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"54 12","pages":""},"PeriodicalIF":4.5000,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Immunology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/eji.202350798","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Sodium lauryl sulfate (SLS) is used as a control irritant in patch testing for allergic contact dermatitis (ACD). However, up to 20% of those tested react to SLS, whereby the pathophysiological basis of this reaction is still unclear. To mimic patch test reactions, we repeatedly applied SLS to the skin of wild-type mice. Reactions were compared with those in a classical ACD model induced by oxazolone and an irritant contact dermatitis (ICD) model induced by croton oil. Skin inflammation was assessed with ear thickness measurements, immunohistochemistry, qRT-PCR, and flow cytometry. Topical SLS treatment was further investigated in Flg/Hrnr−/−, Myd88/Tlr3−/−, and Rag1−/− mouse models. All three compounds caused ear swelling with different courses. Oxazolone treatment, compared with the ICD model, resulted in a greater influx of immune cells (CD4+, MHCII+, CD11b+). Similarly, SLS did not induce immune cell infiltration or expression of selected inflammatory and regulatory cytokines. SLS induced the most pronounced keratinocyte proliferation. Compared with wild-type mice, topical SLS application did not increase ear swelling in skin barrier deficient Flg/Hrnr−/− mice, but led to significantly delayed swelling in mice with defects in innate or adaptive immune functions (Myd88/Tlr3−/−, Rag1−/−). SLS-induced contact dermatitis differed from classical ACD and ICD, as it elicited less pronounced immune alterations. Skin barrier impairment does not affect SLS-induced contact dermatitis, whereas both innate and adaptive components are involved in SLS skin reactions.

Abstract Image

局部十二烷基硫酸钠的免疫反应不同于传统的刺激性和过敏性接触性皮炎。
在过敏性接触性皮炎(ACD)的贴片测试中,十二烷基硫酸钠(SLS)被用作对照刺激物。然而,多达 20% 的受测者会对 SLS 产生反应,而这种反应的病理生理基础尚不清楚。为了模拟斑贴试验反应,我们在野生型小鼠的皮肤上反复涂抹 SLS。我们将这种反应与草唑酮诱导的经典 ACD 模型和巴豆油诱导的刺激性接触性皮炎 (ICD) 模型中的反应进行了比较。皮肤炎症通过耳厚测量、免疫组化、qRT-PCR 和流式细胞术进行评估。在 Flg/Hrnr-/-、Myd88/Tlr3-/- 和 Rag1-/- 小鼠模型中进一步研究了局部 SLS 治疗。所有三种化合物都会引起耳部肿胀,且持续时间不同。与 ICD 模型相比,奥沙唑酮治疗会导致更多的免疫细胞(CD4+、MHCII+、CD11b+)涌入。同样,SLS 没有诱导免疫细胞浸润或表达特定的炎症和调节细胞因子。SLS 能诱导最明显的角质细胞增殖。与野生型小鼠相比,局部使用 SLS 不会增加皮肤屏障缺陷的 Flg/Hrnr-/- 小鼠的耳部肿胀,但会导致先天性或适应性免疫功能缺陷的小鼠(Myd88/Tlr3-/、Rag1-/-)的耳部肿胀明显延迟。SLS诱导的接触性皮炎不同于传统的ACD和ICD,因为它引起的免疫改变不太明显。皮肤屏障受损不会影响 SLS 诱导的接触性皮炎,而先天性和适应性成分都参与了 SLS 皮肤反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.30
自引率
3.70%
发文量
224
审稿时长
2 months
期刊介绍: The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信