A detailed quantitative analysis of circulating T peripheral and follicular helper lymphocytes in patients with rheumatoid arthritis and systemic lupus erythematosus

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-11-01 DOI:10.1016/j.reuma.2024.07.002

Raquel Sánchez-Gutiérrez , Marlen Vitales-Noyola , Larisa González-Baranda , Diana P. Portales-Pérez , Esther Layseca-Espinosa , Mariana H. García-Hernández , Roberto González-Amaro

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Abstract

Introduction and objective

Peripheral and follicular helper T lymphocytes (Tph and Tfh, respectively) have an important role in B cell immune responses and the pathogenesis of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Although several studies on the number of Tph and Tfh cells in these conditions have been published, different phenotypes have been employed for their analysis. In this study, we assessed the levels and function of Tph and Tfh cells in blood samples from patients with RA and SLE by using an extended immunophenotype.

Materials and methods

In a cross-sectional pilot study, blood samples from twenty-seven patients with RA and fifteen with SLE, and twenty-six healthy controls were studied. The levels of Tph (CD4⁺PD-1⁺CXCR5⁻CD38⁺CD69⁺ICOS⁺) and Tfh (CD4⁺PD-1⁺CXCR5⁺CD38⁺CD69⁺ICOS⁺) cells were analyzed by flow cytometry. In addition, the function of Tph/Tfh cells was estimated by measuring the synthesis of IL-21 by these lymphocytes as well as the number of circulating plasmablasts (CD19⁺CD27⁺CD20⁻CD38^hi).

Results

Increased percentages of Tph and Tfh lymphocytes were detected in patients with RA and SLE. Furthermore, the synthesis of IL-21 tended to be higher in both conditions, and higher levels of plasmablasts were detected in these patients, compared to controls. In patients with SLE, the number of Tph cells was associated with disease activity and with the levels of circulating plasmablasts, whereas in patients with RA a significant correlation between Tph cells and evolution time was observed.

Discussion and conclusions

Our data of Tph and Tfh lymphocytes, based in the analysis of an extended phenotype of these cells, provides further evidence on their involvement in the pathogenesis of RA and SLE.

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对类风湿性关节炎和系统性红斑狼疮患者循环 T 周围和滤泡辅助淋巴细胞的详细定量分析

简介和目的外周和滤泡辅助性T淋巴细胞（分别为Tph和Tfh）在B细胞免疫反应以及类风湿性关节炎（RA）和系统性红斑狼疮（SLE）的发病机制中起着重要作用。尽管已发表了多项关于这些疾病中 Tph 和 Tfh 细胞数量的研究，但分析时采用了不同的表型。在这项研究中，我们使用扩展免疫表型评估了 RA 和系统性红斑狼疮患者血液样本中 Tph 和 Tfh 细胞的水平和功能。材料和方法在一项横断面试验研究中，我们研究了 27 名 RA 患者、15 名系统性红斑狼疮患者和 26 名健康对照者的血液样本。通过流式细胞术分析了 Tph（CD4+PD-1+CXCR5-CD38+CD69+ICOS+）和 Tfh（CD4+PD-1+CXCR5+CD38+CD69+ICOS+）细胞的水平。结果在 RA 和系统性红斑狼疮患者中检测到 Tph 和 Tfh 淋巴细胞百分比增加。此外，与对照组相比，IL-21 在这两种情况下的合成量往往更高，在这些患者中检测到的浆细胞水平也更高。在系统性红斑狼疮患者中，Tph 细胞的数量与疾病活动和循环浆细胞的水平有关，而在 RA 患者中，Tph 细胞与演变时间之间存在显著的相关性。讨论与结论我们对 Tph 和 Tfh 淋巴细胞的数据基于对这些细胞扩展表型的分析，为它们参与 RA 和系统性红斑狼疮的发病机制提供了进一步的证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.