The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia.

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Hao Wu, Yingxue Han, Jikang Liu, Rong Zhao, Shizhen Dai, Yajun Guo, Nan Li, Feng Yang, Shenming Zeng
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引用次数: 0

Abstract

Background: Follicular atresia significantly impairs female fertility and hastens reproductive senescence. Apoptosis of granulosa cells is the primary cause of follicular atresia. Pyroptosis and necroptosis, as additional forms of programmed cell death, have been reported in mammalian cells. However, the understanding of pyroptosis and necroptosis pathways in granulosa cells during follicular atresia remains unclear. This study explored the effects of programmed cell death in granulosa cells on follicular atresia and the underlying mechanisms.

Results: The results revealed that granulosa cells undergo programmed cell death including apoptosis, pyroptosis, and necroptosis during follicular atresia. For the first time, we identified the formation of a PANoptosome complex in porcine granulosa cells. This complex was initially identified as being composed of ZBP1, RIPK3, and RIPK1, and is recruited through the RHIM domain. Additionally, we demonstrated that caspase-6 is activated and cleaved, interacting with RIPK3 as a component of the PANoptosome. Heat stress may exacerbate the activation of the PANoptosome, leading to programmed cell death in granulosa cells.

Conclusions: Our data identified the formation of a PANoptosome complex that promoted programmed cell death in granulosa cells during the process of follicular atresia. These findings provide new insights into the molecular mechanisms underlying follicular atresia.

PANoptosome的组装和激活促进了猪颗粒细胞在卵泡闭锁过程中的程序性细胞死亡。
背景:卵泡闭锁严重影响女性的生育能力,并加速生殖衰老。颗粒细胞凋亡是卵泡闭锁的主要原因。作为程序性细胞死亡的其他形式,哺乳动物细胞中的热凋亡和坏死也有报道。然而,人们对卵泡闭锁过程中颗粒细胞中的热凋亡和坏死途径仍不清楚。本研究探讨了颗粒细胞程序性细胞死亡对卵泡闭锁的影响及其内在机制:结果:研究结果显示,颗粒细胞在卵泡闭锁过程中会发生程序性细胞死亡,包括细胞凋亡、热凋亡和坏死。我们首次在猪颗粒细胞中发现了PANoptosome复合体的形成。初步确定该复合体由 ZBP1、RIPK3 和 RIPK1 组成,并通过 RHIM 结构域被招募。此外,我们还证明了 Caspase-6 被激活和裂解,并与作为 PANoptosome 组成部分的 RIPK3 相互作用。热应激可能会加剧PANoptosome的激活,导致颗粒细胞的程序性细胞死亡:我们的数据发现,在卵泡闭锁过程中,PANoptosome复合物的形成促进了颗粒细胞的程序性细胞死亡。这些发现为了解卵泡闭锁的分子机制提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
10.30
自引率
0.00%
发文量
822
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