Variable effects of transient Wolbachia infections on alphaviruses in Aedes aegypti.

IF 3.4 2区医学 Q1 PARASITOLOGY

PLoS Neglected Tropical Diseases Pub Date : 2024-11-04 DOI:10.1371/journal.pntd.0012633

Brittany L Dodson, Sujit Pujhari, Marco Brustolin, Hillery C Metz, Jason L Rasgon

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引用次数: 0

Abstract

Wolbachia pipientis (= Wolbachia) has promise as a tool to suppress virus transmission by Aedes aegypti mosquitoes. However, Wolbachia can have variable effects on mosquito-borne viruses. This variation remains poorly characterized, yet the multimodal effects of Wolbachia on diverse pathogens could have important implications for public health. Here, we examine the effects of transient somatic infection with two strains of Wolbachia (wAlbB and wMel) on the alphaviruses Sindbis virus (SINV), O'nyong-nyong virus (ONNV), and Mayaro virus (MAYV) in Ae. aegypti. We found variable effects of Wolbachia including enhancement and suppression of viral infections, with some effects depending on Wolbachia strain. Both wAlbB- and wMel-infected mosquitoes showed enhancement of SINV infection rates one week post-infection, with wAlbB-infected mosquitoes also having higher viral titers than controls. Infection rates with ONNV were low across all treatments and no significant effects of Wolbachia were observed. The effects of Wolbachia on MAYV infections were strikingly strain-specific; wMel strongly blocked MAYV infections and suppressed viral titers, while wAlbB had more modest effects. The variable effects of Wolbachia on vector competence underscore the importance of further research into how this bacterium impacts the virome of wild mosquitoes including the emergent human pathogens they transmit.

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埃及伊蚊瞬时沃尔巴克氏体感染对阿尔法病毒的不同影响。

Wolbachia pipientis（=沃尔巴克氏体）有望成为抑制埃及伊蚊传播病毒的工具。然而，沃尔巴克氏菌对蚊媒病毒的影响各不相同。这种变化的特征还很不明显，但狼杆菌对不同病原体的多模式效应可能对公共卫生有重要影响。在这里，我们研究了埃及姬蚊瞬时体细胞感染两株沃尔巴克氏体（wAlbB 和 wMel）对α-病毒信比斯病毒（SINV）、欧宁宁病毒（ONNV）和玛雅洛病毒（MAYV）的影响。我们发现沃尔巴克氏菌有不同的作用，包括增强和抑制病毒感染，其中一些作用取决于沃尔巴克氏菌菌株。感染了 wAlbB 和 wMel 的蚊子在感染后一周的 SINV 感染率都有所提高，感染了 wAlbB 的蚊子的病毒滴度也高于对照组。在所有处理中，ONNV 的感染率都很低，没有观察到狼杆菌的显著影响。沃尔巴克氏体对 MAYV 感染的影响具有明显的菌株特异性；wMel 强烈阻止 MAYV 感染并抑制病毒滴度，而 wAlbB 的影响则较为温和。沃尔巴克氏菌对病媒能力的不同影响强调了进一步研究这种细菌如何影响野生蚊子病毒组（包括它们传播的新出现的人类病原体）的重要性。

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来源期刊

PLoS Neglected Tropical Diseases PARASITOLOGY-TROPICAL MEDICINE

自引率

10.50%

发文量

723

期刊介绍： PLOS Neglected Tropical Diseases publishes research devoted to the pathology, epidemiology, prevention, treatment and control of the neglected tropical diseases (NTDs), as well as relevant public policy. The NTDs are defined as a group of poverty-promoting chronic infectious diseases, which primarily occur in rural areas and poor urban areas of low-income and middle-income countries. Their impact on child health and development, pregnancy, and worker productivity, as well as their stigmatizing features limit economic stability. All aspects of these diseases are considered, including: Pathogenesis Clinical features Pharmacology and treatment Diagnosis Epidemiology Vector biology Vaccinology and prevention Demographic, ecological and social determinants Public health and policy aspects (including cost-effectiveness analyses).