Effectiveness of a combination of laccase and green coffee extract on oral malodor: a comparative, randomized, controlled, evaluator-blind, parallel-group trial.

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS
Sylvia L Santos, Caterina Holz, Kimberly Milleman, Jeffery Milleman, Gu Wenqian, Luis R Mateo
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引用次数: 0

Abstract

Oral malodor negatively impacts a person's quality of life and may affect up to 50% of the population. The aim of this randomized, placebo and no-product controlled, evaluator-blind, proof-of-concept study was to evaluate the effectiveness and safety of the single use of two experimental lozenges containing the laccase enzyme and green coffee extract (with and without flavor) in reducing intrinsic oral malodor. Following 12-16 h of avoidance of oral hygiene,156 generally healthy subjects presented at screening and baseline visits with a mean organoleptic odor intensity (OI) score of ⩾2 and an OralChromaTMreading of ⩾125 parts per billion (ppb) hydrogen sulfide (H2S) gas and were randomly assigned to receive either one of the two experimental lozenges, a placebo lozenge, or no-product. Following the supervised use of the assigned products, subjects' oral malodor was evaluated using OI assessments and OralChromaTMmeasurement for volatile sulfur compounds (VSCs) immediately following product use (approximately 5 min), and at 30 min, 1 h, 2 h, 3 h and 4 h. The two experimental lozenges, with and without flavor, showed significant reductions in OI scores compared with the placebo and no-product groups at all time points (p< 0.001). At 5 min post-product use, the experimental lozenges, with and without flavor, were significantly better than the no-product group in reducing the VSCs (p< 0.04). The results of individual VSC components (hydrogen sulfide, methyl mercaptan and dimethyl sulfide) were variable; both experimental lozenges notably reduced hydrogen sulfide and methyl mercaptan levels in most post-use assessments. Four minor adverse events were reported, none of which were directly linked to the product. In conclusion, the experimental lozenges, whether flavored or not, were safe and effective in reducing oral malodor over a span of 4 h, based on organoleptic OI scores.Clinical Trial No: NCT05950529.

结合使用漆酶和绿咖啡提取物对口腔异味的疗效:随机对照、评估者盲法、平行组比较试验。
口腔异味会对人的生活质量产生负面影响,影响人群可能高达 50%。这项随机、安慰剂和无产品对照、评估者盲法、概念验证研究的目的是评估单次使用两种含有漆酶和绿咖啡提取物(含香精和不含香精)的实验性润喉糖在减少内在口腔异味方面的有效性和安全性。在避免口腔卫生12至16小时后,156名一般健康的受试者在筛查和基线检查时,其平均感官气味强度(OI)评分≥2,OralChromaTM读数≥十亿分之125(ppb)硫化氢(H2S)气体,并被随机分配接受两种实验性锭剂之一、安慰剂锭剂或无产品。在监督下使用指定产品后,受试者在使用产品后的第一时间(约 5 分钟)以及 30 分钟、1 小时、2 小时、3 小时和 4 小时后,使用 OI 评估和 OralChromaTM 测量挥发性硫化合物 (VSC) 来评估受试者的口腔异味情况。与安慰剂组和无产品组相比,两种实验性含片(含香精和不含香精)在所有时间点的 OI 评分均显著降低(p < 0.001)。在产品使用后 5 分钟,含香精和不含香精的实验菱形糖在降低 VSC 方面明显优于不含产品组(p < 0.04)。单个 VSC 成分(硫化氢、甲硫醇和二甲基硫醚)的结果各不相同;在大多数使用后评估中,两种实验性润喉糖都明显降低了硫化氢和甲硫醇的水平。报告了四起轻微的不良事件,其中没有一起与产品直接相关。总之,根据感官 OI 评分,实验性润喉糖无论是否有香精,在 4 小时内都能安全有效地减少口腔异味。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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