Magnetic 3D bioprinting of skeletal muscle spheroid for a spheroid-based screening assay

IF 2.7 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

SLAS Discovery Pub Date : 2024-10-28 DOI:10.1016/j.slasd.2024.100190

Chayanit Chaweewannakorn , Khin The Nu Aye , Joao N. Ferreira

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引用次数: 0

Abstract

Over the past decade, there has been a rapid development in the use of magnetic three dimensional (3D) based cell culture systems. Concerning the skeletal muscle, 3D culture systems can provide biological insights for translational clinical research in the fields of muscle physiology and metabolism. These systems can enhance the cell culture environment by improving spatially-oriented cellular assemblies and morphological features closely mimicking the in vivo tissues/organs, since they promote strong interactions between cells and the extracellular matrix (ECM). However, the time-consuming and complex nature of 3D traditional culture techniques pose a challenge to the widespread adoption of 3D systems. Herein, a bench protocol is presented for creating an innovative, promptly assembled and user-friendly culture platform for the magnetic 3D bioprinting of skeletal muscle spheroids. Our protocol findings revealed consistent morphological outcomes and the functional development of skeletal muscle tissue, as evidenced by the expression of muscle-specific contractile proteins and myotubes and the responsiveness to stimulation with cholinergic neurotransmitters. This proof-of-concept protocol confirmed the future potential for further validation and application of spheroid-based assays in human skeletal muscle research.

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磁性骨骼肌球体三维生物打印，用于基于球体的筛选测定。

在过去十年中，基于三维（3D）的磁性细胞培养系统得到了快速发展。关于骨骼肌，三维培养系统可为肌肉生理学和新陈代谢领域的转化临床研究提供生物学见解。由于三维培养系统能促进细胞与细胞外基质（ECM）之间的强烈相互作用，因此能通过改善空间导向的细胞集结和形态特征来模拟体内组织/器官，从而改善细胞培养环境。然而，三维传统培养技术的耗时和复杂性对三维系统的广泛应用构成了挑战。本文介绍了一种工作台方案，用于创建一个创新、快速组装和用户友好的培养平台，用于骨骼肌球体的磁性三维生物打印。我们的方案研究结果表明，骨骼肌组织的形态学结果和功能发育是一致的，肌肉特异性收缩蛋白和肌管的表达以及对胆碱能神经递质刺激的反应都证明了这一点。这一概念验证方案证实了未来在人体骨骼肌研究中进一步验证和应用基于球蛋白的检测方法的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

SLAS Discovery Chemistry-Analytical Chemistry

CiteScore

7.00

自引率

3.20%

发文量

审稿时长

39 days

期刊介绍： Advancing Life Sciences R&D: SLAS Discovery reports how scientists develop and utilize novel technologies and/or approaches to provide and characterize chemical and biological tools to understand and treat human disease. SLAS Discovery is a peer-reviewed journal that publishes scientific reports that enable and improve target validation, evaluate current drug discovery technologies, provide novel research tools, and incorporate research approaches that enhance depth of knowledge and drug discovery success. SLAS Discovery emphasizes scientific and technical advances in target identification/validation (including chemical probes, RNA silencing, gene editing technologies); biomarker discovery; assay development; virtual, medium- or high-throughput screening (biochemical and biological, biophysical, phenotypic, toxicological, ADME); lead generation/optimization; chemical biology; and informatics (data analysis, image analysis, statistics, bio- and chemo-informatics). Review articles on target biology, new paradigms in drug discovery and advances in drug discovery technologies. SLAS Discovery is of particular interest to those involved in analytical chemistry, applied microbiology, automation, biochemistry, bioengineering, biomedical optics, biotechnology, bioinformatics, cell biology, DNA science and technology, genetics, information technology, medicinal chemistry, molecular biology, natural products chemistry, organic chemistry, pharmacology, spectroscopy, and toxicology. SLAS Discovery is a member of the Committee on Publication Ethics (COPE) and was published previously (1996-2016) as the Journal of Biomolecular Screening (JBS).