Gastric microbiome composition accompanied with the Helicobacter pylori related DNA methylation anomaly.

IF 3 4区医学 Q2 GENETICS & HEREDITY

Epigenomics Pub Date : 2024-11-04 DOI:10.1080/17501911.2024.2418803

Takuya Shijimaya, Tomomitsu Tahara, Tsubasa Shimogama, Jumpei Yamazaki, Sanshiro Kobayashi, Naohiro Nakamura, Yu Takahashi, Takashi Tomiyama, Toshiro Fukui, Makoto Naganuma

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引用次数: 0

Abstract

Aim: DNA methylation is associated with gastric cancer and Helicobacter pylori (H. pylori) infection, while increasing evidence indicated involvement of other microbes reside in gastric mucosa during gastric tumorigenesis. We investigated bacterial communities in the gastric mucosa accompanied with H. pylori related methylation anomaly.Materials & methods: Gastric mucosa samples from antrum were obtained from 182 cancer-free patients. Bacterial communities were evaluated using 16S rRNA sequencing. The result was correlated with H. pylori related promoter CpG island (CGI) methylation of five genes (IGF2, SLC16A12, SOX11, P2RX7 and MYOD1), LINE1 hypomethylation and telomere length.Results & conclusion: We showed correlation between lower bacterial alpha diversity and higher CGI methylation. Multivariate analysis demonstrated older age (t = 3.46, p = 0.0007), H. pylori infection (t = 9.99, p < 0.0001) and lower bacterial alfa diversity (Shannon index: t = -2.34, p = 0.02) were significantly associated with CGI hypermethylation. In genus or family levels, increased abundance of Helicobacter was associated with hyper CGI methylation with strongest correlation, while decreased abundance of four bacteria (Intrasporangiaceae family, Macellibacteroides, Peptostreptococcus and Dietziaceae family) was also associated with hyper CGI methylation. Our findings suggest the potential correlation between CGI methylation induction and lower bacterial alpha diversity in the gastric mucosa accompanied by H. pylori infection.

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胃微生物组的组成与幽门螺旋杆菌相关的 DNA 甲基化异常有关。

目的：DNA 甲基化与胃癌和幽门螺杆菌（H. pylori）感染有关，而越来越多的证据表明，胃粘膜中的其他微生物也参与了胃肿瘤的发生。我们研究了胃粘膜中与幽门螺杆菌相关的甲基化异常的细菌群落：从 182 名无癌症患者的胃黏膜取样。使用 16S rRNA 测序对细菌群落进行评估。结果与幽门螺杆菌相关的五个基因（IGF2、SLC16A12、SOX11、P2RX7 和 MYOD1）启动子 CpG 岛（CGI）甲基化、LINE1 低甲基化和端粒长度相关：我们发现细菌α多样性较低与CGI甲基化程度较高之间存在相关性。多变量分析表明，年龄较大（t = 3.46，p = 0.0007）、幽门螺杆菌感染（t = 9.99，p = 0.02）与 CGI 高甲基化显著相关。在属或科的层面上，螺旋杆菌数量的增加与 CGI 高甲基化的相关性最强，而四种细菌（孢子内囊菌科、Macellibacteroides、Peptostreptococcus 和 Dietziaceae 科）数量的减少也与 CGI 高甲基化相关。我们的研究结果表明，CGI 甲基化诱导与幽门螺杆菌感染导致的胃黏膜细菌α多样性降低之间存在潜在的相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Epigenomics GENETICS & HEREDITY-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

>12 weeks

期刊介绍： Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.