Discovery of five diagnostic biomarkers associated with immune cell infiltration in cases of acute myocardial infarction.

IF 1.4 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS

Minerva cardiology and angiology Pub Date : 2024-11-04 DOI:10.23736/S2724-5683.24.06542-6

Qiushi Suo, Pengfei Li, Zhiping Liu, Chuncheng Qu, Hanbing Song

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Abstract

Background: Acute myocardial infarction (AMI) remains one of the leading causes of mortality and morbidity worldwide.

Methods: GSE61144 and GSE66360 were the sources of microarray gene expression profiles for acute myocardial infarction patients and were acquired from the Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/). After merging the datasets, genes that were differentially expressed were chosen.

Results: A total of 234 genes were found to have different expression levels. Of these, 206 genes were upregulated, and 28 genes were downregulated. Five coexpression modules were identified by WGCNA, with the yellow module showing a high correlation with AMI (r=0.65, P=2.0e-15). Ninety-two hub genes were selected in the yellow module by setting a threshold of module membership (MM) greater than 0.8 and gene significance (GS) higher than 0.4. By overlapping these genes with the differentially expressed genes, 81 hub genes were obtained. Five key genes (C5AR1, CXCL1, CXCL2, FPR1, and P2RY13) were identified through PPI analysis. AMI patients exhibited elevated levels of immune cell infiltration, and immune scores in AMI samples were significantly positively correlated with all five key genes. Moreover, the expression levels of these five genes were higher in AMI patients. These five genes possessed area under the curve (AUC) values exceeding 0.8 for diagnosing AMI, thereby demonstrating their efficacy as diagnostic markers.

Conclusions: C5AR1, CXCL1, CXCL2, FPR1, and P2RY13 have the potential to be useful biomarkers in diagnosing AMI and are linked to immune cell infiltration in AMI, opening up new avenues for future research into the pathogenesis of AMI.

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发现急性心肌梗死病例中与免疫细胞浸润相关的五个诊断生物标志物。

背景：急性心肌梗死（AMI急性心肌梗死（AMI）仍然是全球死亡和发病的主要原因之一：GSE61144 和 GSE66360 是急性心肌梗死患者微阵列基因表达谱的来源，它们来自基因表达总库（GEO）数据库（https://www.ncbi.nlm.nih.gov/geo/）。合并数据集后，筛选出差异表达的基因：结果：共发现 234 个基因有不同的表达水平。结果：共发现 234 个基因有不同的表达水平，其中 206 个基因上调，28 个基因下调。WGCNA 发现了五个共表达模块，其中黄色模块与 AMI 高度相关（r=0.65，P=2.0e-15）。通过设定模块成员（MM）大于 0.8 和基因显著性（GS）大于 0.4 的阈值，在黄色模块中筛选出 92 个枢纽基因。通过将这些基因与差异表达基因重叠，得到了 81 个枢纽基因。通过PPI分析确定了五个关键基因（C5AR1、CXCL1、CXCL2、FPR1和P2RY13）。AMI 患者的免疫细胞浸润水平升高，AMI 样本中的免疫评分与所有五个关键基因均呈显著正相关。此外，这五个基因在 AMI 患者中的表达水平更高。这五个基因在诊断 AMI 时的曲线下面积（AUC）值超过了 0.8，从而证明了它们作为诊断标记物的有效性：结论：C5AR1、CXCL1、CXCL2、FPR1 和 P2RY13 有可能成为诊断 AMI 的有用生物标记物，它们与 AMI 中的免疫细胞浸润有关，为今后研究 AMI 的发病机制开辟了新途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Minerva cardiology and angiology CARDIAC & CARDIOVASCULAR SYSTEMS-

CiteScore

2.60

自引率

18.80%

发文量

118