S-Adenosylmethionine Treatment Diminishes the Proliferation of Castration-Resistant Prostate Cancer Cells by Modulating the Expression of miRNAs.

IF 2.9 4区 医学 Q3 IMMUNOLOGY
Archivum Immunologiae et Therapiae Experimentalis Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI:10.2478/aite-2024-0022
Thomas Schmidt
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引用次数: 0

Abstract

AdoMet (S-adenosylmethionine) inhibits cancer cell proliferation and migration via epigenetic alterations. This study aimed to investigate whether AdoMet may cause alterations in microRNA (miRNA) expression profiles that are important for the initiation and progression of prostate cancer. PC-3 cells were treated with AdoMet before miRNA sequencing. A total of 17 differentially expressed miRNAs were detected. Target gene prediction was performed by means of databases. Results were aligned to transcriptomic data. The bioinformatic analysis revealed upregulation of anticancerogenic genes, downregulation of cancerogenic-related processes and pathways. Knocking down hsa-miR-192-5p in PC-3 cells resulted in downregulation of cancer cell proliferation, thus confirming these results.

S-腺苷蛋氨酸通过调节 miRNAs 的表达减少阉割耐药前列腺癌细胞的增殖
AdoMet(S-腺苷蛋氨酸)通过表观遗传学改变抑制癌细胞的增殖和迁移。本研究旨在探讨 AdoMet 是否会导致微RNA(miRNA)表达谱的改变,而微RNA对前列腺癌的发生和发展具有重要作用。在进行 miRNA 测序之前,先用 AdoMet 处理 PC-3 细胞。共检测到 17 个差异表达的 miRNA。通过数据库对目标基因进行了预测。结果与转录组数据进行了比对。生物信息学分析表明,抗癌基因上调,致癌相关过程和通路下调。在 PC-3 细胞中敲除 hsa-miR-192-5p 会导致癌细胞增殖下调,从而证实了上述结果。
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来源期刊
CiteScore
5.90
自引率
0.00%
发文量
26
审稿时长
>12 weeks
期刊介绍: Archivum Immunologiae et Therapiae Experimentalis (AITE), founded in 1953 by Ludwik Hirszfeld, is a bimonthly, multidisciplinary journal. It publishes reviews and full original papers dealing with immunology, experimental therapy, immunogenetics, transplantation, microbiology, immunochemistry and ethics in science.
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