Light-driven differences in bacterial networks and organic matter decomposition: Insights from an analysis of the harmful cyanobacterium Microcystis aeruginosa PCC 7806

Abstract

Freshwater systems are critical yet often underestimated components of global carbon cycling, functioning both as carbon sinks and sources. Cyanobacteria play a key role in this cycle by capturing atmospheric carbon dioxide through photosynthesis. The captured carbon is either released back into the atmosphere or sequestered in sediments following organismal decay. This study examines the pivotal role of cyanobacteria, specifically Microcystis aeruginosa PCC 7806, in the biogeochemical cycling of carbon in freshwater ecosystems, with a focus on how light influences the degradation of cyanobacteria-derived organic matter. Using a combination of 16S rDNA sequencing and excitation-emission matrix coupled with parallel factor (EEM-PARAFAC) analysis, we conducted a 50-day experiment to investigate the dynamics of dissolved organic matter (DOM) and lysate organic matter (LOM) derived from M. aeruginosa PCC 7806 under light and dark conditions. Our results demonstrate that light significantly impacts bacterial community composition, gene functionality, and the decomposition of organic matter. The findings emphasize the crucial role of light in facilitating microbial adaptation, stabilizing microbial networks and driving organic substrate transformation. These insights underscore the influence of light on microbial community dynamics and organic matter degradation, revealing shifts in microbial populations under varying light conditions. This suggests a strong link between photochemical processes and microbial activity, with significant ecological implications.