Evaluation of pregnancy toxemia in goats: Metabolic profile, hormonal findings, and redox balance

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Murat Uzti̇mür , Cennet Nur Ünal
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Abstract

Pregnancy toxemia entails the development of hyperketonemia due to the negative energy balance that occurs in the last trimester of pregnancy in dairy goats. There are a limited number of studies on new diagnostic biomarkers and the pathophysiology of pregnancy toxemia in goats. This study assessed the hormones spexin and irisin and redox biomarker and metabolic profile changes in goats with pregnancy toxemia. The study included 36 hair goats, with 12 in the subclinical pregnancy toxemia (SPT) group, 12 in the clinical pregnancy toxemia (CPT) group, and 12 in the control group (CG). Spexin, irisin, and insulin hormone concentrations were determined by ELISA, while metabolic profile parameters were evaluated with an automatic chemical analyzer and redox balance by spectroscopy. In the goats with pregnancy toxemia, serum levels of spexin, irisin, insulin, and glucose were significantly lower than those of CG goats. The SPT group had significantly higher ischemia-modified albumin concentrations than CG goats, while the CPT group had significantly lower native thiol concentrations compared to CG animals. The concentrations of beta-hydroxybutyric acid and non-esterified fatty acids in the groups with pregnancy toxemia were significantly higher than those of control animals. In the diagnosis of pregnancy toxemia, spexin had sensitivity of 90 %, specificity of 95 %, and an area under the curve value of 0.988, while irisin had sensitivity of 90 %, specificity of 91 %, and an AUC value of 0.979. In this study, both spexin and irisin hormones showed excellent performance in the diagnosis of pregnancy toxemia. In addition, pregnancy toxemia was found to cause increased oxidative stress and significant changes in metabolic and endocrine metabolism in goats. Measuring these biomarkers in both healthy dairy goats and those with pregnancy toxemia may contribute significantly to the management and diagnosis of herd health.
评估山羊的妊娠毒血症:代谢概况、荷尔蒙结果和氧化还原平衡
妊娠毒血症是指奶山羊在妊娠最后三个月出现能量负平衡而导致的高酮血症。有关山羊妊娠毒血症的新诊断生物标志物和病理生理学的研究数量有限。本研究评估了妊娠毒血症山羊体内的荷尔蒙spexin和鸢尾素以及氧化还原生物标志物和代谢特征的变化。该研究包括36只山羊,其中亚临床妊娠毒血症(SPT)组12只,临床妊娠毒血症(CPT)组12只,对照组(CG)12只。用酶联免疫吸附法测定胰岛素、鸢尾素和胰岛素激素的浓度,用自动化学分析仪评估代谢轮廓参数,用光谱法评估氧化还原平衡。患有妊娠毒血症的山羊血清中的胰岛素、鸢尾素、胰岛素和葡萄糖水平明显低于CG山羊。SPT组的缺血修饰白蛋白浓度明显高于CG山羊,而CPT组的原生硫醇浓度明显低于CG动物。妊娠毒血症组的β-羟丁酸和非酯化脂肪酸浓度明显高于对照组。在妊娠毒血症的诊断中,spexin的灵敏度为90%,特异性为95%,曲线下面积值为0.988,而鸢尾素的灵敏度为90%,特异性为91%,曲线下面积值为0.979。在这项研究中,spexin 激素和鸢尾素激素在诊断妊娠毒血症方面都表现出色。此外,研究还发现妊娠毒血症会导致山羊氧化应激增加,新陈代谢和内分泌代谢发生显著变化。对健康奶山羊和妊娠毒血症奶山羊的这些生物标志物进行测量,可大大有助于管理和诊断羊群健康状况。
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来源期刊
Small Ruminant Research
Small Ruminant Research 农林科学-奶制品与动物科学
CiteScore
3.10
自引率
11.10%
发文量
210
审稿时长
12.5 weeks
期刊介绍: Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.
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