Prostanoid synthesis in isolated parenchymal and nonparenchymal mouse liver cells in the presence of arachioonic acid

Abstract

Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A₂ (TXB₂) of prostacyclin (6-keto PGF_1∝) and one of the prostaglandins (PGF_2∝) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB₂, while smaller amounts of 6-keto PGF_1∝ and PGF_2∝ were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase in TXB₂ production and provoked the synthesis of PGF_2∝ and 6-keto PGF_1∝. Theaddition ofarachidonate to hepatocytes did not influence prostanoid production at concentrations below 10⁻⁵ M. Higher concentrations further increased TXB₂ production and also increased the synthesis of 6-keto PGF_1∝ and PGF_2∝. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10⁻⁷−10⁻⁵M arachidonate increased the TXB₂, 6-keto PGF_1∝ and PGF_2∝ synthesis in nonparenchymal cells. No further increase was found at higher concentrations.