Neutrophils inhibit bone formation by directly contacting osteoblasts and suppressing osteogenic differentiation

IF 3.5 2区医学 Q2 ENDOCRINOLOGY & METABOLISM

Bone Pub Date : 2024-10-28 DOI:10.1016/j.bone.2024.117310

Yijun Liu , Fengyuan Guo , Zhenshuo Han , Ying Yin , Guangjin Chen , Yifan Zhang , Qingming Tang , Lili Chen

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Abstract

Neutrophils have been extensively studied for their critical roles in supporting immune defense mechanisms, initiating bone regeneration, and promoting angiogenesis. Nonetheless, the influence of neutrophils on physiological conditions, particularly in the context of bone development, remains incompletely understood. In this study, we examined the effects of non-inflammatory neutrophils on bone physiology by depleting Ly6G⁺ neutrophils and inducing neutropenia through myelosuppression. Our results demonstrated a notable increase in bone mass and a decrease in the bone marrow cavity upon depletion of the neutrophils. These effects were attributed to the direct interaction between neutrophils and osteoblasts, independent of reduced secretion of typical inflammatory cytokines or diminished osteoclast differentiation. This observation suggests a non-inflammatory function of neutrophils within the endosteal microenvironment, where they regulate osteogenic differentiation to preserve optimal bone mass, shape healthy three-dimensional bone trabecular structures, and create ample space for hematopoietic niche development.

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中性粒细胞通过直接接触成骨细胞和抑制成骨分化来抑制骨形成。

中性粒细胞在支持免疫防御机制、启动骨再生和促进血管生成方面发挥着至关重要的作用，因此人们对其进行了广泛的研究。然而，中性粒细胞对生理状况的影响，尤其是对骨平衡的影响，仍未得到充分了解。在这项研究中，我们通过消耗 Ly6G+ 中性粒细胞并通过骨髓抑制诱导中性粒细胞减少，研究了非炎症性中性粒细胞对骨生理的影响。我们的研究结果表明，消耗成熟的中性粒细胞后，骨量明显增加，骨髓腔缩小。这种效应归因于中性粒细胞与成骨细胞之间的直接相互作用，与典型炎症细胞因子分泌减少或破骨细胞分化减弱无关。这一观察结果表明，中性粒细胞在骨内膜微环境中具有非炎症性功能，它们能调节成骨分化以保持最佳骨量，塑造健康的三维骨小梁结构，并为造血龛发育创造充足的空间。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bone 医学-内分泌学与代谢

CiteScore

8.90

自引率

4.90%

发文量

264

审稿时长

30 days

期刊介绍： BONE is an interdisciplinary forum for the rapid publication of original articles and reviews on basic, translational, and clinical aspects of bone and mineral metabolism. The Journal also encourages submissions related to interactions of bone with other organ systems, including cartilage, endocrine, muscle, fat, neural, vascular, gastrointestinal, hematopoietic, and immune systems. Particular attention is placed on the application of experimental studies to clinical practice.