Conditional deletion of IP3R1 by Islet1-Cre in mice reveals a critical role of IP3R1 in interstitial cells of Cajal in regulating GI motility.

IF 6.9 2区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Hong Wang, Beili Zhao, Lei Huang, Xiangbin Zhu, Na Li, Can Huang, Zhen Han, Kunfu Ouyang
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Abstract

Background and aims: Inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) has been proposed to play a physiological role in regulating gastrointestinal (GI) motility, but the underlying cell-dependent mechanism remains unclear. Here, we utilized cell-specific IP3R1 deletion strategies to address this question in mice.

Methods: Conditional IP3R1 knockout mice using Wnt1-Cre, Islet1-Cre mice, and smMHC-CreEGFP were generated. Cell lineage tracing was performed to determine where gene deletion occurred in the GI tract. Whole-gut transit assay and isometric tension recording were used to assess GI function in vivo and in vitro.

Results: In the mouse GI tract, Islet1-Cre targeted smooth muscle cells (SMCs) and interstitial cells of Cajal (ICCs), but not enteric neurons. IP3R1 deletion by Islet1-Cre (isR1KO) caused a phenotype of intestinal pseudo-obstruction (IPO), evidenced by prolonged whole-gut transit time, enlarged GI tract, abdominal distention, and early lethality. IP3R1 deletion by Islet1-Cre not only reduced the frequency of spontaneous contractions but also decreased the contractile responses to the muscarinic agonist carbachol (CCh) and electrical field stimulation (EFS) in colonic circular muscles. By contrast, smMHC-CreEGFP only targeted SMCs in the mouse GI tract. Although IP3R1 deletion by smMHC-CreEGFP (smR1KO) also reduced the contractile responses to CCh and EFS in colonic circular muscles, the frequency of spontaneous contractions was less affected, and neither global GI abnormalities nor early lethality was found in smR1KO mice.

Conclusions: IP3R1 deletion in both ICCs and SMCs but not in SMCs alone causes an IPO phenotype, suggesting that IP3R1 in ICCs plays an essential role in regulating GI motility in vivo.

通过 Islet1-Cre 小鼠条件性缺失 IP3R1 揭示了 IP3R1 在 Cajal 间质细胞中调节胃肠道运动的关键作用。
背景和目的:1,4,5-三磷酸肌醇受体 1 型(IP3R1)被认为在调节胃肠道(GI)蠕动中发挥生理作用,但其潜在的细胞依赖机制仍不清楚。在此,我们利用细胞特异性 IP3R1 基因缺失策略来解决小鼠的这一问题:方法:利用 Wnt1-Cre、Islet1-Cre 小鼠和 smMHC-CreEGFP 产生了条件性 IP3R1 基因敲除小鼠。方法:利用 Wnt1-Cre 小鼠和 Islet1-Cre 小鼠以及 smMHC-CreEGFP 产生条件性 IP3R1 基因敲除小鼠,并进行细胞系追踪,以确定基因缺失发生在消化道的哪个部位。使用全肠转运试验和等张力记录来评估体内和体外的消化道功能:结果:在小鼠消化道中,Islet1-Cre 以平滑肌细胞(SMCs)和卡雅尔间质细胞(ICCs)为靶细胞,但不以肠神经元为靶细胞。通过 Islet1-Cre 缺失 IP3R1(isR1KO)会导致肠假性梗阻(IPO)表型,表现为全肠转运时间延长、消化道扩大、腹胀和早期死亡。通过 Islet1-Cre 缺失 IP3R1 不仅会降低自发收缩的频率,还会降低结肠圆肌对毒蕈碱激动剂卡巴胆碱(CCh)和电场刺激(EFS)的收缩反应。相比之下,smMHC-CreEGFP 只针对小鼠消化道的 SMC。虽然通过 smMHC-CreEGFP 缺失 IP3R1(smR1KO)也会降低结肠圆肌对 CCh 和 EFS 的收缩反应,但自发收缩的频率受到的影响较小,而且 smR1KO 小鼠既未发现整体消化道异常,也未发现早期致死:结论:在 ICCs 和 SMCs 中同时缺失 IP3R1 而非仅在 SMCs 中缺失 IP3R1 会导致 IPO 表型,这表明 ICCs 中的 IP3R1 在调节体内消化道运动中发挥着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Gastroenterology
Journal of Gastroenterology 医学-胃肠肝病学
CiteScore
12.20
自引率
1.60%
发文量
99
审稿时长
4-8 weeks
期刊介绍: The Journal of Gastroenterology, which is the official publication of the Japanese Society of Gastroenterology, publishes Original Articles (Alimentary Tract/Liver, Pancreas, and Biliary Tract), Review Articles, Letters to the Editors and other articles on all aspects of the field of gastroenterology. Significant contributions relating to basic research, theory, and practice are welcomed. These publications are designed to disseminate knowledge in this field to a worldwide audience, and accordingly, its editorial board has an international membership.
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