Enhanced Fecal Norovirus Detection Using Magneto-Nanocatalys–Based Immunoassay

IF 3.2 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2024-10-29 DOI:10.1002/biot.202400447

Indra Memdi Khoris, Kenta Tsuruga, Jirayu Boonyakida, Enoch Y. Park

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Abstract

A new method has been developed to improve the detection of norovirus (NoV) in complex fecal samples using nanocatalyst-based immunoassays. The method involves using multifunctional trimetallic nanoparticles, known as Ag@Fe₃O₄@Au NPs. These nanoparticles consist of a core of silver (Ag) and a shell of iron oxide (Fe₃O₄) decorated with isolated gold nanoparticles (Au NPs). The nanoparticles have enhanced catalytic activity, making them an ideal nanocatalyst for reducing 4-nitrophenol (4-NP, yellow) to 4-aminophenol (4-AP, colorless). The developed Ag@Fe₃O₄@Au NPs-based immunoassay achieved a limit of detection (LOD) of 1.9 pg/mL for norovirus-like particles (NoV-LP) and 6.97 RNA copy number/mL for fecal NoV. In fecal sample analysis for NoV, a heat treatment at 65°C was necessary to prevent degradation of the target protein, ensuring sensitive detection. This work successfully combined multifunctional nanocatalysts for advanced immunoassays, which could contribute to developing nano-biosensing platforms.

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利用基于磁性纳米催化的免疫测定法加强粪便诺罗病毒检测

我们开发了一种新方法，利用基于纳米催化剂的免疫测定技术改进复杂粪便样本中诺如病毒（NoV）的检测。该方法涉及使用多功能三金属纳米粒子，即 Ag@Fe3O4@Au NPs。这些纳米粒子由银（Ag）核心和氧化铁（Fe3O4）外壳组成，外壳上装饰有孤立的金纳米粒子（Au NPs）。这些纳米颗粒具有更强的催化活性，是将 4-硝基苯酚（4-NP，黄色）还原为 4-氨基苯酚（4-AP，无色）的理想纳米催化剂。所开发的基于 Ag@Fe3O4@Au NPs 的免疫测定对诺如病毒样颗粒（NoV-LP）的检测限（LOD）为 1.9 pg/mL，对粪便 NoV 的检测限（LOD）为 6.97 RNA 拷贝数/mL。在对粪便样本进行诺如病毒分析时，必须在 65°C 下进行热处理，以防止目标蛋白降解，从而确保灵敏检测。这项工作成功地将多功能纳米催化剂结合到先进的免疫测定中，有助于开发纳米生物传感平台。

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.