Exercise improves muscle mitochondrial dysfunction-associated lipid profile under circadian rhythm disturbance.

IF 1.6 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Yu Gu, Dong-Hun Seong, Wenduo Liu, Zilin Wang, Yong Whi Jeong, Jae-Cheol Kim, Dae Ryong Kang, Rose Ji Eun Lee, Jin-Ho Koh, Sang Hyun Kim
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引用次数: 0

Abstract

We investigated whether endurance exercise training (EXT) ameliorates circadian rhythm (CR)-induced risk factors by improving skeletal muscle (SKM) mitochondrial biogenesis, reducing oxidative stress, and modulating apoptotic protein expression. We distinguished between regular and shift workers using the National Health and Nutrition Examination Survey (NHANES) and investigated the health problems caused by shift work (CR disturbance) and the potential therapeutic effects of exercise. In our animal study, 36 rats underwent 12 weeks of CR disturbance, divided into regular and irregular CR groups. These groups were further split into EXT (n = 12) and sedentary (n = 12) for an additional 8 weeks. We analyzed SKM tissue to understand the molecular changes induced by CR and EXT. NHANES data were analyzed using SAS 9.4 and Prism 8 software, while experimental animal data were analyzed using Prism 8 software. The statistical procedures used in each experiment are indicated in the figure legends. Our studies showed that CR disturbance increases dyslipidemia, alters circadian clock proteins (BMAL1, PER2), raises apoptotic protein levels, and reduces mitochondrial biogenesis in SKM. EXT improved LDL-C and HDLC levels without affecting muscle BMAL1 expression. It also enhanced mitochondrial biogenesis (AMPK, PGC-1α, Tfam, NADH-UO, COX-I), antioxidant levels (Catalase, SOD1, SOD2), and apoptotic protein (p53, Bax/Bcl2) expression or activity in SKM. We demonstrated that shift work-induced CR disturbance leads to dyslipidemia, diminished mitochondrial biogenesis, and reduced antioxidant capacity in SKM. However, EXT can counteract dyslipidemia under CR disturbance, potentially lowering the risk of cardiovascular disorders.

在昼夜节律紊乱的情况下,运动可改善肌肉线粒体功能障碍相关的血脂状况。
我们研究了耐力运动训练(EXT)是否能通过改善骨骼肌(SKM)线粒体生物生成、减少氧化应激和调节凋亡蛋白表达来改善昼夜节律(CR)诱发的风险因素。我们利用美国国家健康与营养调查(NHANES)区分了普通工人和轮班工人,并调查了轮班工作(昼夜节律紊乱)导致的健康问题以及运动的潜在治疗效果。在我们的动物研究中,36 只大鼠接受了为期 12 周的 CR 干扰,分为定期 CR 组和不定期 CR 组。这些组又分为EXT组(n = 12)和静坐组(n = 12),共8周。我们对 SKM 组织进行了分析,以了解 CR 和 EXT 引起的分子变化。NHANES 数据使用 SAS 9.4 和 Prism 8 软件进行分析,实验动物数据使用 Prism 8 软件进行分析。每个实验所使用的统计程序均在图例中标明。我们的研究表明,CR 干扰会增加 SKM 的血脂异常、改变昼夜节律钟蛋白(BMAL1、PER2)、提高凋亡蛋白水平并减少线粒体的生物生成。EXT 可改善低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDLC)水平,但不影响肌肉中 BMAL1 的表达。它还增强了 SKM 的线粒体生物生成(AMPK、PGC-1α、Tfam、NADH-UO、COX-I)、抗氧化水平(过氧化氢酶、SOD1、SOD2)和凋亡蛋白(p53、Bax/Bcl2)的表达或活性。我们证明,轮班工作引起的 CR 干扰会导致 SKM 中的血脂异常、线粒体生物生成减弱和抗氧化能力降低。然而,EXT 可以抵消 CR 干扰下的血脂异常,从而降低心血管疾病的风险。
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来源期刊
Korean Journal of Physiology & Pharmacology
Korean Journal of Physiology & Pharmacology PHARMACOLOGY & PHARMACY-PHYSIOLOGY
CiteScore
3.20
自引率
5.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: The Korean Journal of Physiology & Pharmacology (Korean J. Physiol. Pharmacol., KJPP) is the official journal of both the Korean Physiological Society (KPS) and the Korean Society of Pharmacology (KSP). The journal launched in 1997 and is published bi-monthly in English. KJPP publishes original, peer-reviewed, scientific research-based articles that report successful advances in physiology and pharmacology. KJPP welcomes the submission of all original research articles in the field of physiology and pharmacology, especially the new and innovative findings. The scope of researches includes the action mechanism, pharmacological effect, utilization, and interaction of chemicals with biological system as well as the development of new drug targets. Theoretical articles that use computational models for further understanding of the physiological or pharmacological processes are also welcomed. Investigative translational research articles on human disease with an emphasis on physiology or pharmacology are also invited. KJPP does not publish work on the actions of crude biological extracts of either unknown chemical composition (e.g. unpurified and unvalidated) or unknown concentration. Reviews are normally commissioned, but consideration will be given to unsolicited contributions. All papers accepted for publication in KJPP will appear simultaneously in the printed Journal and online.
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