Transcriptomic analysis of sexually dimorphic cypris larvae of the rhizocephalan barnacle Peltogasterella gracilis

Abstract

Peltogasterella gracilis (Cirripedia: Rhizocephala), a crustacean parasite of hermit crabs, displays genotypic sex determination. Its larvae are planktonic, and female larvae settle on the host. Subsequently, the females control the host's behavior by spreading a root-like structure called “the interna” within the host's body, and form sacs containing eggs called “the externae” outside the host's body. On the other hand, male larvae settle on immature externae and become dwarf males. The cypris larvae of P. gracilis show sexual dimorphism in size and morphology. However, there is no understanding of the molecular mechanisms underlying the sexual dimorphism observed at the larval stage. Here, we conducted a transcriptome analysis and compared the expression of genes in male and female cyprids to better understand their sexual differentiation and settlement processes. A total of 2870 differentially expressed transcripts, comprising 456 female- and 2414 male-biased transcripts were identified. Among the male-biased ones, ionotropic glutamate receptor-, heat shock protein-, acetylcholine-, and homeobox-, cuticle-related transcripts were included. Additionally, 29 gene ontology terms were associated with the sex-specific traits. The present study improves our understanding of sex determination, sexual differentiation, and settlement processes of rhizocephalans.