Cerebellar activity in PINK1 knockout rats during volitional gait.

IF 4.1 Q1 CLINICAL NEUROLOGY
Brain communications Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI:10.1093/braincomms/fcae249
Valerie DeAngelo, Justin D Hilliard, Chia-Han Chiang, Jonathan Viventi, George C McConnell
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Abstract

Preclinical models of Parkinson's disease are imperative to gain insight into the neural circuits that contribute to gait dysfunction in advanced stages of the disease. A PTEN-induced putative kinase 1 knockout early-onset model of Parkinson's disease may be a useful rodent model to study the effects of neurotransmitter degeneration caused by a loss of PTEN-induced putative kinase 1 function on brain activity during volitional gait. The goal of this study was to measure changes in neural activity at the cerebellar vermis at 8 months of age. It was found that gait deficits, except run speed, were not significantly different from age-matched wild-type controls, as previously reported. PTEN-induced putative kinase 1 knockout (n = 4) and wild-type (n = 4) rats were implanted with a micro-electrocorticographic array placed over cerebellar vermis Lobules VI (a-c) and VII. Local field potential recordings were obtained during volitional gait across a runway. Power spectral analysis and coherence analysis were used to quantify network oscillatory activity in frequency bands of interest. Cerebellar vermis power was hypoactive in the beta (VIb, VIc and VII) and alpha (VII) bands at cerebellar vermis Lobules VIb, VIc and VII in PTEN-induced putative kinase 1 knockout rats compared with wild-type controls during gait (P < 0.05). These results suggest that gait improvement in PTEN-induced putative kinase 1 knockout rats at 8 months may be a compensatory mechanism attributed to movement corrections caused by a decreased inhibition of the alpha band of cerebellar vermis Lobule VII and beta band of Lobules VIb, VIc and VII. The PTEN-induced putative kinase 1 knockout model may be a valuable tool for understanding the circuit mechanisms underlying gait dysfunction in patients with early-onset Parkinson's disease with a functional loss of PTEN-induced putative kinase 1. Future studies investigating the cerebellar vermis as a potential biomarker and therapeutic target for the treatment of gait dysfunction in Parkinson's disease are warranted.

PINK1 基因敲除大鼠在意志步态过程中的小脑活动。
要深入了解导致帕金森病晚期步态功能障碍的神经回路,就必须建立帕金森病的临床前模型。PTEN诱导的假定激酶1基因敲除早期帕金森病模型可能是研究PTEN诱导的假定激酶1功能缺失导致神经递质变性对自主步态过程中大脑活动影响的有用啮齿类动物模型。本研究的目的是测量8个月大时小脑蚓部神经活动的变化。结果发现,除奔跑速度外,步态缺陷与年龄匹配的野生型对照组无显著差异。在小脑蚓部第六小叶(a-c)和第七小叶上植入微型皮层电图阵列,PTEN诱导的推定激酶1基因敲除大鼠(n = 4)和野生型大鼠(n = 4)均被植入该阵列。局部场电位记录是在大鼠自愿走过跑道时获得的。功率谱分析和相干分析用于量化相关频段的网络振荡活动。与野生型对照组相比,PTEN诱导的推定激酶1基因敲除大鼠在步态过程中,小脑蚓部小叶VIb、VIc和VII的β(VIb、VIc和VII)和α(VII)频段的功率较低(P < 0.05)。这些结果表明,PTEN诱导的推定激酶1基因敲除大鼠在8个月时步态的改善可能是一种代偿机制,归因于小脑蚓部第七小叶的α带和第六b、第六c和第七小叶的β带抑制作用减弱导致的运动矫正。PTEN诱导的假定激酶1基因敲除模型可能是了解早期帕金森病患者因PTEN诱导的假定激酶1功能缺失而导致步态功能障碍的电路机制的重要工具。未来有必要将小脑蚓部作为治疗帕金森病步态功能障碍的潜在生物标志物和治疗靶点进行研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
7.00
自引率
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审稿时长
6 weeks
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